ADMINISTRATION OF WARFARIN ACCELERATES THE RECOVERY IN ISCHEMIA/REPERFUSION-INDUCED ACUTE PANCREATITIS
ADMINISTRATION OF WARFARIN ACCELERATES THE RECOVERY IN ISCHEMIA/REPERFUSION-INDUCED ACUTE PANCREATITIS
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DOI：
10.26402/jpp.2020.3.13
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发表时间：
2020-06-01
影响因子：
2.2
通讯作者：
Warzecha, Z.
中科院分区：
文献类型：
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作者：
Maduzia, D.;Ceranowicz, P.;Warzecha, Z.
急性胰腺炎与凝血功能的激活有关，凝血功能与疾病的严重程度密切相关。抗凝剂如肝素或醋硝香豆素的管理已被证明可以降低急性胰腺炎的严重程度，并加速恢复。本研究的目的是确定华法林给药对缺血/再灌注诱导的急性胰腺炎病程的影响。采用胰腺缺血再灌注的方法制备大鼠急性胰腺炎模型。每天一次灌胃给予溶媒（1 ml/剂）或华法林（45、90或180 μ g/kg/剂，溶于1 ml溶媒中）。在胰腺再灌注开始后24 h给予华法林首次剂量。在胰腺再灌注开始后2、5、9和14天评估急性胰腺炎的严重程度。华法林治疗可减少胰腺损伤并加速组织学检查的恢复，这种作用伴随着胰腺消化酶、脂肪酶和淀粉酶的血清活性更快降低。此外，华法林导致促炎性白细胞介素-1 β的血清浓度和D-二聚体的血浆水平较早降低。这些作用与胰腺血流的改善有关。我们的结论是，华法林表现出对胰腺缺血再灌注引起的急性胰腺炎的治疗作用。
Acute pancreatitis is associated with activation of coagulation and there is a close relationship between coagulation and the severity of this disease. Administration of anticoagulants such as heparin or acenocoumarol has shown to reduce the severity of acute pancreatitis and accelerate the recovery. The aim of the current study was to determine the impact of warfarin administration on the course of ischemia/reperfusion-induced acute pancreatitis. Acute pancreatitis was induced in rats by pancreatic ischemia followed by reperfusion. Vehicle (1 ml/dose) or warfarin (45, 90 or 180 mu g/kg/dose in 1 ml of vehicle) were administered intragastrically once a day. The first dose of warfarin was given 24 h after the start of pancreatic reperfusion. The severity of acute pancreatitis was assessed 2, 5, 9 and 14 days after the beginning of pancreatic reperfusion. Treatment with warfarin reduces pancreatic damage and accelerates recovery in histological examination and this effect is accompanied by a faster reduction in serum activity of pancreatic digestive enzymes, lipase and amylase. In addition, warfarin led to an earlier decrease in serum concentration of pro-inflammatory interleukin-1 beta and plasma level of D-dimer. These effects were associated with an improvement of pancreatic blood flow. We conclude that warfarin exhibits a therapeutic effect in acute pancreatitis evoked by pancreatic ischemia followed by reperfusion.