Evaluation of antibacterial textile covered by layer-by-layer coating and loaded with chlorhexidine for wound dressing application
Evaluation of antibacterial textile covered by layer-by-layer coating and loaded with chlorhexidine for wound dressing application
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DOI：
10.1016/j.msec.2019.03.044
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发表时间：
2019-07-01
影响因子：
7.9
通讯作者：
Blanchemain, Nicolas
中科院分区：
文献类型：
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作者：
Aubert-Viard, Francois;Mogrovejo-Valdivia, Alejandra;Blanchemain, Nicolas
本研究的目的是设计一种能够释放氯己定（CHX）作为防腐剂的伤口敷料，确保愈合过程中持久的抗菌效果。首次采用壳聚糖（CHT）与吉尼平（Gpn）交联的方法对纺织无纺布（聚对苯二甲酸乙二醇酯）（PET）进行了改性。优化了试剂（Gpn和CHT）浓度、交联时间和工作温度等参数，以达到最大正电荷表面密度。然后通过一层一层（LbL）沉积由甲基- β -环糊精聚合物（PCD）（阴离子）和CHT（阳离子）组成的多层体系来处理该载体。经过热处理以稳定LbL薄膜后，纺织品被装载CHX作为防腐剂。研究了热处理对细胞相容性的影响，对多层体系降解的影响，对CHX吸附和释放特性的影响，以及对织物抗菌活性的影响。
The aim of this work is to design a wound dressing able to release chlorhexidine (CHX) as antiseptic agent, ensuring long-lasting antibacterial efficacy during the healing. The textile nonwoven (polyethylene terephthalate) (PET) of the dressing was first modified by chitosan (CHT) crosslinked with genipin (Gpn). Parameters such as the concentration of reagents (Gpn and CHT) but also the crosslinking time and the working temperature were optimized to reach the maximal positive charges surface density. This support was then treated by the layer-by-layer (LbL) deposition of a multilayer system composed of methyl-beta-cyclodextrin polymer (PCD) (anionic) and CHT (cationic). After a thermal treatment to stabilize the LbL film, the textiles were loaded with CHX as antiseptic agent. The influence of the thermal treatment i) on the cytocompatibility, ii) on the degradation of the multilayer system, iii) on CHX sorption and release profiles and iv) on the antibacterial activity of the loaded textiles was studied.