Quantitative characterization of visual response properties in the mouse dorsal lateral geniculate nucleus
Quantitative characterization of visual response properties in the mouse dorsal lateral geniculate nucleus
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DOI：
10.1152/jn.00699.2003
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发表时间：
2003-12-01
影响因子：
2.5
通讯作者：
Thompson, ID
中科院分区：
文献类型：
--
作者：
Grubb, MS;Thompson, ID
我们定量分析了野生型C57Bl/6J小鼠膝状背外侧核（dLGN）单个神经元的视觉反应特性。在氟烷和氧化亚氮麻醉下，对小鼠的单个dLGN细胞进行细胞外记录。在利用对闪方刺激的反向相关反应绘制这些细胞的感受野（RFs）后，我们使用正弦光栅来描述它们的空间和线性、空间频率调谐、时间频率调谐和对比响应特性。我们样本中的所有细胞的RFs都由一个单一的、大致圆形的“中心”机制主导，该机制对刺激亮度的增加（ON-center）或减少（OFF-center）作出反应，并且几乎所有细胞都通过了空间总和线性度的修正零检验。利用高斯差分模型将空间频率调谐与细胞rf的空间特性联系起来，发现小鼠dLGN细胞具有较大的rf（中心直径约为11度），相应的空间分辨率较差（约为0.2c/度）。从时间上看，小鼠dLGN中的大多数细胞对约4hz的刺激反应最好。我们在小鼠dLGN中寻找平行处理的证据，发现它只存在于ON- center和OFF-center细胞之间的功能差异中：ON- center细胞比OFF-center细胞对刺激对比更敏感。
We present a quantitative analysis of the visual response properties of single neurons in the dorsal lateral geniculate nucleus (dLGN) of wild-type C57Bl/6J mice. Extracellular recordings were made from single dLGN cells in mice under halothane and nitrous oxide anesthesia. After mapping the receptive fields (RFs) of these cells using reverse correlation of responses to flashed square stimuli, we used sinusoidal gratings to describe their linearity of spatial summation, spatial frequency tuning, temporal frequency tuning, and contrast response characteristics. All cells in our sample had RFs dominated by a single, roughly circular "center" mechanism that responded to either increases (ON-center) or decreases (OFF-center) in stimulus luminance, and almost all cells passed a modified null test for linearity of spatial summation. A difference of Gaussians model was used to relate spatial frequency tuning to the spatial properties of cells' RFs, revealing that mouse dLGN cells have large RFs (center diameter approximately 11degrees) and correspondingly poor spatial resolution (approximately 0.2c/degrees). Temporally, most cells in the mouse dLGN respond best to stimuli of approximately 4 Hz. We looked for evidence of parallel processing in the mouse dLGN and found it only in a functional difference between ON- and OFF-center cells: ON- center cells were more sensitive to stimulus contrast than their OFF-center neighbors.