Degradation activity of Clostridium species DC-1 in the presence of indigenous michoorganisms and Escherichia coli.
Degradation activity of Clostridium species DC-1 in the presence of indigenous michoorganisms and Escherichia coli.
复制标题
本地微生物和大肠杆菌存在下梭状芽胞杆菌 DC-1 的降解活性。
DOI：
10.1007/s10163-012-0060-2
复制
发表时间：
2012
影响因子：
3.1
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Bhowmik;A.;Ishimura;K.;Nakamura;K. and Takamizawa;K.
我们研究了梭菌DC-1与其它细菌联合作用下的顺-1，2-二氯乙烯（cis-1，2-DCE）脱氯能力。变性梯度凝胶电泳结果显示，在cis-1，2-DCE降解过程中，DC-1为优势带型，其它梭菌为优势带型。添加大肠杆菌（Escherichia coli，E.大肠杆菌（E. coli）对DC-1的脱氯活性没有影响。杆菌用DC-1和E.大肠杆菌中梭菌的优势导致E.在生物修复状态下共生生长。这一结果表明，梭菌属DC-1作为cis-1，2-DCE的降解剂的可能性，在cis-1，2-DCE污染的场地，土著微生物群落存在。用E.大肠杆菌表明，污染场地中的菌株对cis-1，2-DCE的降解没有抑制作用，在降解过程中，其它梭菌占优势，E.大肠杆菌会减少。这一发现可能是一个非常积极的方法，在脂肪族氯化组分污染的场地实施脱氯细菌。
We showed thecis-1,2-dichloroethylene (cis-1,2-DCE) dechlorination ability ofClostridiumspecies DC-1 in association with other bacteria. Result of denaturing gradient gel electrophoresis showed the dominant band pattern of DC-1 during the degradation time ofcis-1,2-DCE and dominance of some otherClostridiumspecies. Experiment with addition ofEscherichia coli(E. coli) showed that dechlorinating activity of DC-1 was not inhibited by the presence ofE. coli. Pour plate experiment with DC-1 andE. colirevealed that the dominance ofClostridiumspecies caused the decrease ofE. coligrowth in a bioremediation state. This result suggested the possibility ofClostridiumspecies DC-1 as a degrader ofcis-1,2-DCE, in acis-1,2-DCE contaminated site where an indigenous microbial community is present. Experiment conducting withE. colisuggested that the strain in the contaminated site did not inhibit the degradation ofcis-1,2-DCE and during the degradation period, rather some otherClostridiumspecies became dominant and the growth ofE. coliwould be decreased. This finding could be a very positive approach for implementing the dechlorinating bacteria at aliphatic chlorinated component contaminated sites.