Well Plate Microfluidic System for Investigation of Dynamic Platelet Behavior Under Variable Shear Loads
Well Plate Microfluidic System for Investigation of Dynamic Platelet Behavior Under Variable Shear Loads
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DOI：
10.1002/bit.23243
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发表时间：
2011-12-01
影响因子：
3.8
通讯作者：
Nevill, J. Tanner
中科院分区：
文献类型：
--
作者：
Conant, Carolyn G.;Schwartz, Michael A.;Nevill, J. Tanner
在受控的剪切应力下实时研究血小板行为提供了对许多血管疾病的潜在机制的见解，并能够评估以血小板为中心的治疗方法。研究均匀剪切流下血管壁血小板行为的两种最常用的方法是平行板流动室和锥板粘度计。通常，这些方法难以使用，缺乏实验灵活性，提供低数据含量，通量低，并且需要大的试剂体积。在这里，我们报告了一种基于孔板微流体（WPM）的系统，该系统以易于使用的孔板格式提供高通量、低试剂消耗和高实验灵活性。该系统由具有集成微流体通道阵列的孔板、气动接口、自动显微镜和软件组成。该WPM系统用于研究剪切应力下的动态血小板行为。介绍了多通道设计，并对全血进行了剪切载荷测试，以确定其在研究血栓形成方面的适用性。使用正常生理剪切（0.1-20 dyn/cm（2））和病理剪切（20-200 dyn/cm（2））装置研究血小板在各种剪切、基质涂层和单层条件下的体外行为。高生理相关性、低血液消耗和增加的通量为血管生物学研究人员创造了一种有价值的技术。该方法还可扩展到其他研究领域，包括炎症，癌症生物学和发育/干细胞研究。Biotechnol. Bioeng. 2011; 108：2978-2987. (C)2011 Wiley Periodicals，Inc.
The study of platelet behavior in real-time under controlled shear stress offers insights into the underlying mechanisms of many vascular diseases and enables evaluation of platelet-focused therapeutics. The two most common methods used to study platelet behavior at the vessel wall under uniform shear flow are parallel plate flow chambers and cone-plate viscometers. Typically, these methods are difficult to use, lack experimental flexibility, provide low data content, are low in throughput, and require large reagent volumes. Here, we report a well plate microfluidic (WPM)-based system that offers high throughput, low reagent consumption, and high experimental flexibility in an easy to use well plate format. The system consists of well plates with an integrated array of microfluidic channels, a pneumatic interface, an automated microscope, and software. This WPM system was used to investigate dynamic platelet behavior under shear stress. Multiple channel designs are presented and tested for shear loads with whole blood to determine their applicability to study thrombus formation. Normal physiological shear (0.1-20 dyn/cm(2)) and pathological shear (20-200 dyn/cm(2)) devices were used to study platelet behavior in vitro under various shear, matrix coating, and monolayer conditions. The high physiological relevance, low blood consumption, and increased throughput create a valuable technique available to vascular biology researchers. The approach also has extensibility to other research areas including inflammation, cancer biology, and developmental/stem cell research. Biotechnol. Bioeng. 2011; 108: 2978-2987. (C) 2011 Wiley Periodicals, Inc.