Cellular responses induced by silver nanoparticles:: In vitro studies
Cellular responses induced by silver nanoparticles:: In vitro studies
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DOI：
10.1016/j.toxlet.2008.04.009
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发表时间：
2008-06-30
影响因子：
3.5
通讯作者：
Paknikar, K. M.
中科院分区：
文献类型：
--
作者：
Arora, S.;Jain, J.;Paknikar, K. M.
作为我们实验室正在进行的项目的一部分，我们对 7-20 nm 球形银纳米颗粒 (SNP) 与 HT-1080 和 A431 细胞的体外相互作用进行了系统研究，以开发用于治疗烧伤创面感染的局部抗菌剂。暴露于 SNP（高达 6.25 μg/mL）后，两种细胞类型的形态保持不变。然而，在较高浓度（6.25-50 μg/mL）下，细胞变得不那么多面体，更加纺锤形、皱缩和圆形。 XTT测定显示HT-1080和A431的IC50值分别为10.6和11.6μg/mL。当细胞受到类似于 1/2 IC50 浓度的 SNP (6.25 μg/mL) 攻击时，氧化应激的明显迹象，即 GSH 降低（与 HT-1080 中相似，2.5 倍，与 A431 中相似 2 倍）和 SOD（与 HT-1080 中相似，1.6 倍，A431 中相似，3 倍）以及增加观察到脂质过氧化（与 HT-1080 中的 2.5 倍相似，与 A431 中的 2 倍相似）。 A431 细胞中过氧化氢酶和 GPx 水平的变化在两种细胞类型中均无统计学意义。 SNP 暴露细胞中的 DNA 碎片表明细胞凋亡。当用 caspase-3 测定法监测 SNP 的凋亡阈值时，发现细胞凋亡开始所需的浓度（HT-1080 中为 0.78 μg/mL，A431 中为 1.56 μg/mL）远低于坏死浓度（两种细胞类型中为 12.5 μg/mL）。经过适当的体内研究后，这些结果可用于确定 SNP 作为局部抗菌剂的预期应用的安全范围。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
A systematic study on the in vitro interactions of 7-20 nm spherical silver nanoparticles (SNP) with HT-1080 and A431 cells was undertaken as a part of an on-going program in our laboratory to develop a topical antimicrobial agent for the treatment of burn wound infections. Upon exposure to SNP (up to 6.25 mu g/mL), morphology of both the cell types remained unaltered. However, at higher concentrations (6.25-50 mu g/mL) cells became less polyhedral, more fusiform, shrunken and rounded. IC50 values for HT-1080 and A431 as revealed by XTT assay were 10.6 and 11.6 mu g/mL, respectively. When the cells were challenged with similar to 1/2 IC50 concentration of SNP (6.25 mu g/mL), clear signs of oxidative stress, i.e. decreased GSH (similar to 2.5-folds in HT-1080, similar to 2-folds in A431) and SOD (similar to 1.6-folds in HT-1080, 3-folds in A431) as well as increased lipid peroxidation (similar to 2.5-folds in HT-1080, similar to 2-folds in A431) were seen. Changes in the levels of catalase and GPx in A431 cells were statistically insignificant in both cell types. DNA fragmentation in SNP-exposed cells suggested apoptosis. When the apoptotic thresholds of SNP were monitored with caspase-3 assay the concentrations required for the onset of apoptosis were found to be much lower (0.78 mu g/mL in HT-1080, 1.56 mu g/mL in A431) than the necrotic concentration (12.5 mu g/ml, in both cell types). These results can be used to define a safe range of SNP for the intended application as a topical antimicrobial agent after appropriate in vivo studies. (C) 2008 Elsevier Ireland Ltd. All rights reserved.