Synergistic effect of PDGF and FGF2 for cell proliferation and hair inductive activity in murine vibrissal dermal papilla in vitro
Synergistic effect of PDGF and FGF2 for cell proliferation and hair inductive activity in murine vibrissal dermal papilla in vitro
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DOI：
10.1016/j.jdermsci.2015.04.007
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发表时间：
2015-08-01
影响因子：
4.6
通讯作者：
Okochi, Hitoshi
中科院分区：
文献类型：
--
作者：
Kiso, Masahiro;Hamazaki, Tatsuo S.;Okochi, Hitoshi
背景：毛乳头是由一小簇间充质细胞组成，称为毛乳头细胞（dermal papilla cells，DPC）。DPC与表皮细胞密切相互作用，在毛囊发育和毛发周期中产生毛囊和毛干。毛原细胞是治疗脱发的理想细胞来源。然而，一旦DPC被放入传统的二维培养条件下，他们很快就失去了他们的能力，以产生hair follicle.Objective：我们的目的是扩大足够大的人口的DPC，保留他们的头发inductive activity.Methods：小鼠DPC的血小板衍生生长因子-AA（PDGF-AA）和成纤维细胞生长因子2（FGF 2）的存在下培养。结果：FGF 2能显著增加体外培养的触须毛原细胞中血小板源性生长因子受体α（PDGFR α）的表达，并能促进毛囊生长。PDGF-AA是PDGFRa的配体，当与FGF 2一起使用时，协同促进DPC的增殖，并增强DPC中几种卵泡相关基因的表达。毛发再生实验表明，PDGF-AA和FGF-2联合处理的毛原细胞比FGF 2单独处理的毛原细胞具有更好的毛发诱导活性。结论：FGF 2和PDGF-AA的协同作用维持了小鼠毛原细胞的增殖和毛发诱导活性。(C)2015年日本皮肤病研究学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Background: The dermal papilla is composed of a small clump of mesenchymal cells, called dermal papilla cells (DPCs). DPCs closely interact with epidermal cells to give rise to hair follicles and shafts during hair follicle development and the hair cycle. DPCs are promising cell sources for hair regeneration therapy for alopecia patients. However, once DPCs are put into conventional two-dimensional culture conditions, they quickly lose their capability to produce hair follicles.Objective: We aimed to expand a sufficiently large population of DPCs that retain their hair inductive activity.Methods: Murine DPCs were cultured in the presence of platelet-derived growth factor-AA (PDGF-AA) and fibroblast growth factor 2 (FGF2). Expressions of follicular-related genes were analyzed by real time PCR and hair inductive activity was determined by patch assay and chamber assay in vivo.Results: FGF2 significantly increased the expression of platelet-derived growth factor receptor alpha (PDGFR alpha) in cultured vibrissal DPCs. PDGF-AA, a ligand of PDGFRa, promoted proliferation of DPCs synergistically when utilized with FGF2 and enhanced the expression of several follicular-related genes in DPCs. Hair reconstitution assays revealed that DPCs treated with both PDGF-AA and FGF-2 were able to maintain their hair inductive activity better than those treated with FGF2 alone.Conclusion: Both cell proliferation and hair inductive activity in murine DPCs are maintained by the synergistic effect of FGF2 and PDGF-AA. (C) 2015 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.