Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
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DOI：
10.1083/jcb.130.1.67
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发表时间：
1995-07
期刊：
影响因子：
--
通讯作者：
Wheelock MJ
中科院分区：
文献类型：
--
作者：
Knudsen KA;Soler AP;Johnson KR;Wheelock MJ
钙粘蛋白是Ca（2+）依赖性的细胞表面糖蛋白，参与细胞-细胞粘附。在细胞外，跨膜钙粘蛋白如E-、P-和N-钙粘蛋白自缔合，而在细胞内，它们与基于肌动蛋白的细胞骨架间接相互作用。几种称为连环蛋白的细胞内蛋白质，包括α-连环蛋白、β-连环蛋白和斑珠蛋白，与这些钙粘蛋白紧密结合，并用于将它们连接到细胞骨架。在这里，我们提供的证据表明，在成纤维细胞中，α-辅肌动蛋白（而不是纽蛋白）与N-钙粘蛋白/连环蛋白复合物广泛共定位。这与上皮细胞相反，在上皮细胞中，细胞骨架蛋白与E-钙粘蛋白和连环蛋白广泛共定位。我们进一步表明，α-辅肌动蛋白，而不是黏着斑蛋白，coimmunoprecipitates特异性与α-和β-连环蛋白从N-和E-钙粘蛋白表达细胞，但只有当α-连环蛋白存在。此外，我们发现α-辅肌动蛋白与N-钙粘蛋白/连环蛋白复合物以肌动蛋白非依赖性方式共免疫沉淀。因此，我们建议，钙粘蛋白/连环蛋白复合物连接到肌动蛋白细胞骨架通过α-辅肌动蛋白和α-连环蛋白之间的直接关联。
Cadherins are Ca(2+)-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, transmembrane cadherins such as E- , P-, and N-cadherin self-associate, while intracellularly they interact indirectly with the actin-based cytoskeleton. Several intracellular proteins termed catenins, including alpha-catenin, beta- catenin, and plakoglobin, are tightly associated with these cadherins and serve to link them to the cytoskeleton. Here, we present evidence that in fibroblasts alpha-actinin, but not vinculin, colocalizes extensively with the N-cadherin/catenin complex. This is in contrast to epithelial cells where both cytoskeletal proteins colocalize extensively with E-cadherin and catenins. We further show that alpha- actinin, but not vinculin, coimmunoprecipitates specifically with alpha- and beta-catenin from N- and E-cadherin-expressing cells, but only if alpha-catenin is present. Moreover, we show that alpha-actinin coimmunoprecipitates with the N-cadherin/catenin complex in an actin- independent manner. We therefore propose that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between alpha-actinin and alpha-catenin.