Intracellular pH monkey embryos at various stages of organogenesis estimated by dimethadione distribution.
Intracellular pH monkey embryos at various stages of organogenesis estimated by dimethadione distribution.
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通过二甲二酮分布估算器官发生各个阶段的猴胚胎细胞内 pH 值。
DOI：
10.1071/rd9960911
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Nau,H
中科院分区：
文献类型：
--
作者：
Collins,MD;ScottJr,WJ;Hendrickx,AG;Peterson,PE;Nau,H
先前使用14 C-DMO（5，5-二甲基恶唑烷-2，4-二酮或通常称为二甲双酮）经胎盘分布的实验已经证明，大鼠胚胎和液体的pH值在器官发生过程中逐渐降低。本实验的目的是类似地评价食蟹猴器官发生期间的pH变化，食蟹猴是人类胚胎发生的模型。使用通过气相色谱-质谱法定量的DMO（与放射性标记化合物的计数相反），测定食蟹猴胚胎在大致相当的发育期（妊娠第24-36天）内胚胎pHi发生相似的降低。食蟹猴绒毛膜液中的DMO与母体血浆中的DMO的比率也显示出随着胎龄的增加而降低，表明pH值降低。食蟹猴中DMO经胎盘分布显著慢于啮齿类动物。本研究表明，食蟹猴器官形成期间胚胎细胞和胚外液中pH值降低的幅度与啮齿动物胚胎和体液中检测到的类似，但母体血液pH值和胚胎细胞内pH值之间的相对梯度不同。这两个物种之间pH梯度的差异可能导致外源性和内源性物质的差异性经胎盘分布。
Previous experiments using the transplacental distribution of 14C-DMO (5,5-dimethyloxazolidine-2,4-dione or commonly known as dimethadione) have demonstrated that the pH of rat embryos and fluids progressively decreases during organogenesis. The aim of the present experiments was to similarly evaluate pH changes during organogenesis in the cynomolgus monkey, which is a model for human embryogenesis. Using DMO quantitated by gas chromatography-mass spectrometry as opposed to the counting of radiolabelled compound, cynomolgus monkey embryos were determined to undergo a similar decrease in embryonic pHi over an approximately comparable period of development (Days 24-36 of gestation). The ratio of DMO in chorionic fluid to DMO in maternal plasma in the cynomolgus monkey also displayed a decrease with advancing gestational age indicative of a pH decrease. The DMO transplacental distribution was found to be significantly slower in the cynomolgus monkey than that in rodents. The present investigation indicates that the magnitude of the reduction of pH in embryonic cells and in extra-embryonic fluids over a period of organogenesis in the cynomolgus monkey is similar to the reduction detected in rodent embryos and fluids over a comparable developmental period, but the relative gradient between maternal blood pH and embryonic intracellular pH is different. The difference in the pH gradient between the two species may lead to differential transplacental distribution of exogenous and endogenous substances.