Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes
Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes
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DOI：
10.1023/a:1014897607670
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发表时间：
2002-03-01
影响因子：
5.1
通讯作者：
Conti, S
中科院分区：
文献类型：
--
作者：
Tuberosa, R;Sanguineti, MC;Conti, S
我们研究了玉米在水培条件下测定的种子根性状的数量性状基因座(QTL)与水分充足(GY-WW)和水分胁迫(GY-WS)条件下的籽粒产量QTL以及计算为GY-WS/GY-WW的耐旱性指数(DTI)之间的重叠。水培中，初生根长度(R1L)、初生根直径(R1D)、初生根重(R1W)和不定子根重(R2W)分别检测到11个、7个、9个和10个QTL。田间定位GY-WW、GY-WS和DTI的QTL分别为7个、8个和9个。尽管水培根系性状与GY-WW、GY-WS和DTI之间的相关性较弱，但在相应的QTL之间观察到了明显的重叠。R2W的QTL最频繁且一致地与GY-WW、GY-WS和/或DTI的QTL重叠。在4个QTL区域，R2W的增加与GY-WW、GY-WS和/或DTI呈正相关。1号染色体上位于pGAMCTA205和php20644之间的10 cM距离对R1L、R1D、R2W、GY-WW、GY-WS和DTI的影响最大。这些结果表明，利用水培技术在玉米生长早期定位控制根系性状并影响田间GY的QTL区域是可行的。对本文所确定的QTL区域与先前研究不同玉米群体根系性状的QTL区域的比较分析表明，存在许多共同的QTL。
We investigated the overlap among quantitative trait loci (QTLs) in maize for seminal root traits measured in hydroponics with QTLs for grain yield under well-watered (GY-WW) and water-stressed (GY-WS) field conditions as well as for a drought tolerance index (DTI) computed as GY-WS/GY-WW. In hydroponics, 11, 7, 9, and 10 QTLs were identified for primary root length (R1L), primary root diameter (R1D), primary root weight (R1W), and for the weight of the adventitious seminal roots (R2W), respectively. In the field, 7, 8, and 9 QTLs were identified for GY-WW, GY-WS, and DTI, respectively. Despite the weak correlation of root traits in hydroponics with GY-WW, GY-WS, and DTI, a noticeable overlap between the corresponding QTLs was observed. QTLs for R2W most frequently and consistently overlapped with QTLs for GY-WW, GY-WS, and/or DTI. At four QTL regions, an increase in R2W was positively associated with GY-WW, GY-WS, and/or DTI. A 10 cM interval on chromosome 1 between PGAMCTA205 and php20644 showed the strongest effect on R1L, R1D, R2W, GY-WW, GY-WS, and DTI. These results indicate the feasibility of using hydroponics in maize to identify QTL regions controlling root traits at an early growth stage and also influencing GY in the field. A comparative analysis of the QTL regions herein identified with those described in previous studies investigating root traits in different maize populations revealed a number of QTLs in common.