Characterisation of Lactobacillus fermentum SM-7 isolated from koumiss, a potential probiotic bacterium with cholesterol-lowering effects
Characterisation of Lactobacillus fermentum SM-7 isolated from koumiss, a potential probiotic bacterium with cholesterol-lowering effects
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从马奶酒中分离的发酵乳杆菌 SM-7 的特性，一种具有降低胆固醇作用的潜在益生菌
DOI：
10.1002/jsfa.4214
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发表时间：
2011-02-01
影响因子：
4.1
通讯作者：
Yan, Yu Ting
中科院分区：
文献类型：
--
作者：
Dao Dong Pan;Zeng, Xiao Qun;Yan, Yu Ting
背景技术背景：结果：从酸马奶酒中分离得到10株乳酸菌，经筛选得到发酵乳杆菌SM-7，其降胆固醇率为66.8%。它还表现出对酸和胆汁的耐受性以及对致病性土氏大肠杆菌和金黄色葡萄球菌的抗菌活性。发酵乳杆菌SM-7细胞同化61.5%的胆固醇，共沉淀和吸收38.5%的培养基中的胆固醇。胆固醇与胆酸的共沉淀在低于6的pH水平下迅速增加。在体内实验中，使用L. SM-7能显著降低ICR小鼠血清总胆固醇、总甘油三酯、低密度脂蛋白胆固醇和动脉粥样硬化指数（P < 0.01），而对高密度脂蛋白胆固醇无显著影响（P > 0.05）。SM-7组的体重和肝脏重量/体重比低于高胆固醇饮食的小鼠（未给予乳酸杆菌）。实验组小鼠肝、脾、肾组织均未发现细菌移位。发酵菌SM-7是一种具有降胆固醇作用的潜在益生菌。(C)2010年化学工业协会
BACKGROUND: Lactic acid bacteria (LAB) have been demonstrated to have cholesterol-reducing effects in many studies.RESULTS: Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%. It also showed acid and bile tolerance as well as antimicrobial activity against pathogenic Escherchia coli and Staphylococcus aureus. Lactobacillus fermentum SM-7 cells assimilated 61.5% and co-precipitated and absorbed 38.5% of the cholesterol in the media. Co-precipitation of cholesterol with cholic acid increased rapidly at pH levels below 6. In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol and total triglyceride levels, low-density lipoprotein cholesterol concentrations and atherogenic index (P < 0.01), while serum high-density lipoprotein cholesterol concentrations did not increase significantly (P > 0.05). The body weight and liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli. There was no bacterial translocation in the liver, spleen or kidney of experimental mice.CONCLUSION: The results suggested that L. fermentum SM-7 is a potential probiotic bacterium with cholesterol-lowering effects. (C) 2010 Society of Chemical Industry