Isolation of Trypanosoma (Megatrypanum) theileri from dairy cattle in Taiwan.
Isolation of Trypanosoma (Megatrypanum) theileri from dairy cattle in Taiwan.
复制标题
从台湾奶牛中分离出特氏锥虫（Megatrypanum）。
DOI：
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发表时间：
2010
影响因子：
1.2
通讯作者：
Y. Chiu
中科院分区：
文献类型：
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作者：
Yen;Ching;N. Lin;Shih;K. Tung;Y. Chiu
泰氏锥虫（Megatrypanum）是一种牛的血液寄生虫，虽然在世界各地广泛传播，但在台湾从未有报道。当直接涂抹来自发热奶牛的抗凝血样本时，未观察到寄生虫。然而，一个高度独特的形态学特征的锥虫出现在婴儿仓鼠肾（BHK）细胞培养接种非抛出血液血沉棕黄层。观察了该分离株锥虫的不同发育阶段和典型的超微结构。该分离株随后被鉴定为T.通过种特异性PCR（Tth625）、18SrDNA序列比对和核糖体基因内转录间隔区（ITS）标记进行系统发育分析。第一个T。台湾的泰氏分离株（TWTth 1）在BHK细胞培养中可定期传代一年以上，并保持良好的再冻存活力。BHK培养方法对于诊断分离和维持该寄生虫的长期发育是极好的。
Although Trypanosoma (Megatrypanum) theileri, a blood parasite of bovid species, is spread widely throughout the world, it has never been reported in Taiwan. When an anti-coagulated blood sample from febrile dairy cattle was directly smeared, no parasite was observed. However, a highly distinctive morphological feature of trypanosome appeared in baby hamster kidney (BHK) cell culture inoculated with non-thrown blood buffy coat. The different stages and typical ultrastructures of trypanosome were observed in our isolate. The isolate was subsequently identified as T. theileri by species-specific PCR assay (Tth625), 18S rDNA sequencing alignment and internal transcribed spacer of ribosomal genes (ITS) as a marker for molecular phylogenetic analysis. The first T. theileri isolate in Taiwan (TWTth1) could be periodically passaged in BHK cell culture for more than one year and retained good re-cryopreservation viability. The BHK culture method would be excellent for diagnostic isolation and maintenance long-term development of this parasite.
等位基因不平衡和等位基因频率分布作为社会和人口历史的函数。
影响因子：
9.8
作者：
Thompson,EA;Neel,JV
通讯作者：
Neel,JV