The 5'-3' exoribonuclease Pacman is required for normal male fertility and is dynamically localized in cytoplasmic particles in Drosophila testis cells.
The 5'-3' exoribonuclease Pacman is required for normal male fertility and is dynamically localized in cytoplasmic particles in Drosophila testis cells.
复制标题
DOI：
10.1042/bj20071720
复制
发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
M. V. Zabolotskaya;Dominic P. Grima;Ming-Der Lin;T. Chou;S. Newbury
中科院分区：
文献类型：
--
作者：
M. V. Zabolotskaya;Dominic P. Grima;Ming-Der Lin;T. Chou;S. Newbury
核糖核酸外切酶Xrn 1被广泛认为是5 '-3' RNA降解途径中的关键组分。这种酶在酵母和人类之间高度保守，并且已知参与RNA干扰和microRNA的降解以及RNA周转。在酵母和人类组织培养细胞中，Xrn 1已被证明是P体（加工体）的一个组成部分，动态细胞质颗粒可以发生RNA降解。在本文中，我们首次表明，Pacman，果蝇同源的Xrn 1，定位于果蝇睾丸细胞的细胞质颗粒。这些颗粒既存在于有丝分裂的原始干细胞衍生的精原细胞中，也存在于转录活跃的精母细胞中。Pacman与去帽激活剂dDcp 1和解旋酶Me 31 B（Dhh 1同源物）在这些颗粒中共定位，尽管这种共定位不完全重叠，表明这些颗粒内有不同的隔室。含有Pacman的颗粒以与酵母P-体相同的方式响应于应激和5 '-3'衰变因子的耗尽，因此可能是mRNA降解或储存的位点。Pacman被证明是正常果蝇精子发生所必需的，这表明mRNA稳定性的控制在睾丸分化途径中至关重要。
The exoribonuclease Xrn1 is widely recognised as a key component in the 5'-3' RNA degradation pathway. This enzyme is highly conserved between yeast and humans and is known to be involved in RNA interference and degradation of microRNAs as well as RNA turnover. In yeast and human tissue culture cells, Xrn1 has been shown to be a component of P-bodies (processing bodies), dynamic cytoplasmic granules where RNA degradation can take place. In this paper we show for the first time that Pacman, the Drosophila homologue of Xrn1, is localized in cytoplasmic particles in Drosophila testis cells. These particles are present in both the mitotically dividing spermatogonia derived from primordial stem cells and in the transcriptionally active spermatocytes. Pacman is co-localized with the decapping activator dDcp1 and the helicase Me31B (a Dhh1 homologue) in these particles, although this co-localization is not completely overlapping, suggesting that there are different compartments within these granules. Particles containing Pacman respond to stress and depletion of 5'-3' decay factors in the same way as yeast P-bodies, and therefore are likely to be sites of mRNA degradation or storage. Pacman is shown to be required for normal Drosophila spermatogenesis, suggesting that control of mRNA stability is crucial in the testis differentiation pathway.