Functional characterization of alternatively spliced human SERCA3 transcripts.
Functional characterization of alternatively spliced human SERCA3 transcripts.
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选择性剪接的人类 SERCA3 转录本的功能表征。
DOI：
10.1152/ajpcell.1998.275.6.c1449
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Lytton,J
中科院分区：
文献类型：
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作者：
Poch,E;Leach,S;Snape,S;Cacic,T;MacLennan,DH;Lytton,J
肌质（或内质）网Ca 2 +-ATP酶（SERCA）-3已被牵连在可能的失调的Ca 2+稳态，伴随着高血压和糖尿病的病理。我们报告的分子克隆的两个选择性剪接的成绩单从人类SERCA 3基因，ATP 2A 3，编码的蛋白质，不同的羧基末端由36个氨基酸。SERCA 3转录本在淋巴组织、肠、胰腺和前列腺中表达最丰富。由选择性剪接转录本编码的两种人SERCA 3蛋白被单克隆抗体PL/IM 430识别，并表现出Ca 2+摄取和ATP酶活性，其表观Ca 2+亲和力比其他SERCA基因产物低0.5 pCa单位。SERCA 3蛋白的亚细胞分布与SERCA 2b蛋白的亚细胞分布没有区别，在整个细胞的核膜和内质网中表达。分离了两种变体SERCA 3构建体huS 3-I和huS 3-II，其编码具有三个氨基酸差异的蛋白质：Ala-673（在huS 3-I中）取代Thr（在huS 3-II中），Ile-817取代Met，以及Glu-994的插入。huS 3-I显示出比huS 3-II低10倍的转运Ca 2+的能力。
The sarcoplasmic (or endoplasmic) reticulum Ca2+-ATPase (SERCA)-3 has been implicated in the possible dysregulation of Ca2+homeostasis that accompanies the pathology of hypertension and diabetes. We report the molecular cloning of two alternatively spliced transcripts from the human SERCA3 gene,ATP2A3, that encode proteins that differ at their carboxy termini by 36 amino acids. SERCA3 transcripts were most abundantly expressed in lymphoid tissues, intestine, pancreas, and prostate. The two human SERCA3 proteins encoded by alternatively spliced transcripts were recognized by the monoclonal antibody PL/IM430 and demonstrated Ca2+uptake and ATPase activity with an apparent Ca2+affinity 0.5 pCa unit lower than that of other SERCA gene products. The subcellular distribution of SERCA3 protein was indistinguishable from that of SERCA2b, with expression in the nuclear envelope and in the endoplasmic reticulum throughout the cell. Two variant SERCA3 constructs, huS3-I and huS3-II, were isolated that encode proteins with three amino acid differences: Ala-673 (in huS3-I) substituted for Thr (in huS3-II), Ile-817 substituted for Met, and an insertion of Glu-994. huS3-I displayed a 10-fold lower capacity to transport Ca2+than huS3-II.