Surfactant supplementation to enhance the production of vitamin K2 metabolites in shake flask cultures using Escherichia sp. mutant FM3-1709.
Surfactant supplementation to enhance the production of vitamin K2 metabolites in shake flask cultures using Escherichia sp. mutant FM3-1709.
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发表时间：
2014
影响因子：
2.4
通讯作者：
Liu Yan;Zhiming Zheng;Hongwei Qiu;Genhai Zhao;W. Peng;Liu Hui;Wang Li;Zhe-min Li;Hefang Wu
中科院分区：
文献类型：
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作者：
Liu Yan;Zhiming Zheng;Hongwei Qiu;Genhai Zhao;W. Peng;Liu Hui;Wang Li;Zhe-min Li;Hefang Wu
在大肠杆菌深层培养中，研究了添加各种表面活性剂对细胞生长和维生素 K2 代谢产物（如细胞内甲基萘醌 4 (MK-4)、细胞外 MK-4、细胞内 MK-6 和细胞外 MK-6）生产的影响。所有添加的表面活性剂都会引起指数相的延长。甜菜碱、聚氧乙烯油醚和Tween-80有利于大肠杆菌的细胞生长。突变株FM3-1709。最高的细胞生长速度（Xmax）、生物量生产率（Qx）和生物量产率（Yx/s）分别为（12.6±0.2）g/L、（0.21±0.01）g/（L·h）和（2.42±0.02）g/g。结果表明，所有表面活性剂的添加均导致细胞内MK-4的产量降低，而细胞外MK-4的产量显着增加。在所研究的五种表面活性剂中，添加非离子表面活性剂聚氧乙烯油醚（1.0 g/L）导致细胞外MK-4（（33.6±0.4）mg/L）、MK-6（（2.56±0.07）mg/L）产量最高，总MK-4（（47.6±0.4）mg/L）和MK-6产量最高。 ((6.0±0.1)mg/L)。事实证明，添加聚氧乙烯油基醚比MK-6更有利于MK-4的分泌。
The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfactants caused the extension of the exponential phase. Betaine, polyoxyethylene oleyl ether and Tween-80 were favourable to the cell growth of Escherichia sp. mutant strain FM3-1709. The highest cell growth (Xmax), biomass production rate (Qx) and biomass yield (Yx/s) were (12.6±0.2) g/L, (0.21±0.01) g/(L·h) and (2.42± 0.02) g/g, respectively. The results show that the addition of all surfactants led to a lower production of intracellular MK-4, whereas the production of extracellular MK-4 increased remarkably. Among the five investigated surfactants, the addition of the nonionic surfactant polyoxyethylene oleyl ether (1.0 g/L) led to the highest production of extracellular MK-4 ((33.6±0.4) mg/L), MK-6 ((2.56±0.07) mg/L) and the highest yield of total MK-4 ((47.6±0.4) mg/L) and MK-6 ((6.0±0.1) mg/L). The addition of polyoxyethylene oleyl ether proved to be more beneficial for the secretion of MK-4 than MK-6.