Expression of neurotrophin receptor trkB in rat cochlear hair cells at time of rearrangement of innervation
Expression of neurotrophin receptor trkB in rat cochlear hair cells at time of rearrangement of innervation
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DOI：
10.1007/s004410050545
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发表时间：
1996-03-01
影响因子：
3.6
通讯作者：
Zenner, HP
中科院分区：
文献类型：
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作者：
Knipper, M;Zimmermann, U;Zenner, HP
在大鼠出生后耳蜗发育过程中监测高亲和神经营养因子受体trkB的时空分布。trkB免疫反应性除了在假定的传入I型侧枝、传入II型纤维和传出纤维中表达外，trkB免疫反应性也短暂地出现在感觉毛细胞本身中，从出生后5-9天开始为基转，从出生后9-13天开始为尖转。将trkB与传入和传出纤维中表达的p75（NGFR）和传出纤维中表达的GAP-43和synaptophysin进行比较，发现trkB受体在毛细胞中的表达与其神经支配的重排存在时空相关性。神经营养因子受体及其配体的共表达已被提出在功能上参与调节不依赖于靶源性神经营养因子的神经元的存活。因此，trkB在靶毛细胞中的存在表明，自动/旁分泌机制在神经连接重排的关键时期发挥了作用。
The spatio-temporal distribution of the high-affinity neurotrophin receptor trkB was monitored during postnatal development of the rat cochlea. In addition to expression in presumptive afferent type I collaterals, afferent type II fibers, and efferent fibers, trkB immunoreactivity also transiently appeared in the sensory hair cells themselves, from postnatal days 5-9 in the basal turn, and from postnatal days 9-13 in the apical turn. A comparison of trkB with p75(NGFR), which is expressed in afferent and efferent fibers, and GAP-43 and synaptophysin, which are expressed in efferent fibers, revealed a time/space correlation of trkB receptor expression in hair cells with the rearrangement of their innervation. Co-expression of the neurotrophin receptor and its ligand has been proposed to be functionally involved in regulating the survival of neurons independent of target-derived neurotrophin factor. Thus, the presence of trkB in target hair cells, suggests that auto/paracrine mechanisms play a role during this critical period of rearrangement of neural connections.