Extraembryonic venous obstructions lead to cardiovascular malformations and can be embryolethal
Extraembryonic venous obstructions lead to cardiovascular malformations and can be embryolethal
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DOI：
10.1016/s0008-6363(98)00218-1
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发表时间：
1999-01-01
影响因子：
10.8
通讯作者：
Poelmann, RE
中科院分区：
文献类型：
--
作者：
Hogers, B;DeRuiter, MC;Poelmann, RE
目的：为了扩大我们对胎盘血流对人类心脏发育的影响的认识，我们使用了一个胚胎鸡模型，其中胚胎外血流被操纵。方法：首先结扎其中一条卵黄静脉，用墨汁观察血流。通过这种方式，我们可以研究不同结扎位置对心内血流模式的影响。其次，用微夹将卵黄静脉永久结扎，直到心脏分离完成，然后对心脏进行形态学评估。这样，我们可以研究结扎位置对心脏畸形严重程度和发生频率的影响。结合这些结果，我们能够研究不同的心内血流模式对心脏发育的影响。结果：虽然每条静脉结扎导致不同的心内血流模式，但长期结扎在幸存者中导致类似的心血管畸形。这些主要包括室间隔缺损（VSDs）、半月瓣异常和咽弓动脉畸形。结扎位置与心血管畸形发生率比较差异无统计学意义（p < 0.05）。然而，剪断左侧卵黄外侧静脉后的死亡率明显高于对照组(p
Objective: To expand our knowledge concerning the effect of placental blood flow on human heart development, we used an embryonic chicken model in which extraembryonic blood flow was manipulated. Methods: First, one of the three major vitelline veins was ligated, while blood flow was visualized with Indian ink. In this way, we could study the effect of different ligation positions on intracardiac flow patterns. Secondly, these vitelline veins were ligated permanently with a microclip until cardiac septation was completed, thereafter, the hearts were morphologically evaluated. In this way, we could study the impact of the ligation position on the severity and frequency of heart malformations. On combining the results, we were able to study the effect of different intracardiac flow patterns on heart development. Results: Although ligation of each vein resulted in different intracardiac flow patterns, long-term ligation resulted in similar cardiovascular malformations in survivors. These consisted mainly of ventricular septum defects (VSDs), semilunar valve anomalies, and pharyngeal arch artery malformations. There was no significant difference (p>0.05) between the ligation position and the incidence of cardiovascular malformations. However, the percentage mortality after clipping the left lateral vitelline vein was significantly higher (p