Cloning and molecular characterization of fructose-1,6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum
Cloning and molecular characterization of fructose-1,6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum
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DOI：
10.1007/s00299-009-0702-6
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发表时间：
2009-06-01
影响因子：
6.2
通讯作者：
Zhang, Yanlin
中科院分区：
文献类型：
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作者：
Fan, Wei;Zhang, Zhili;Zhang, Yanlin
海马齿（Sesuvium portulacastrum）是一种生长于海滨的红树林植物，是一种耐盐耐旱的盐生植物。目前已对其耐盐抗旱生理结构特征进行了研究，但其分子机制和关键成分尚未明确。在此，果糖-1，6-二磷酸醛缩酶基因，命名为SpFBA，是从S. portulacastrum根对海水的反应。SpFBA cDNA全长1452 bp，开放阅读框为1071 bp，编码357个氨基酸残基的前体蛋白，与其它植物的I类FBA具有高度同源性。半定量RT-PCR分析表明，SpFBA在根中的表达量高于茎和叶，海水、NaCl、ABA和PEG等非生物刺激均能诱导SpFBA的表达。2 - 12 h左右即可生根。过量生产重组SpFBA导致转基因大肠杆菌对盐的耐受性增加。这些结果表明，SpFBA在响应盐胁迫及相关非生物刺激，提高盐藻存活能力方面具有重要作用。马齿苋在高盐和干旱条件下生长。
Sesuvium portulacastrum, a mangrove plant from seashore, is a halophyte species well adapted to salinity and drought. Some efforts have been made to describe its physiological and structural characteristics on salt and drought-tolerance, but the underlying molecular mechanism and key components have not yet been identified. Here, a fructose-1,6-bisphosphate aldolase gene, designated SpFBA, was isolated and characterized from S. portulacastrum roots in response to seawater. The SpFBA cDNA has a total length of 1452 bp with an open reading frame of 1071 bp, and is predicted to encode a precursor protein of 357 amino acid residues sharing high degree of homology with class I FBAs from other plants. Semi-quantitative RT-PCR analysis indicated that the SpFBA was more strongly expressed in roots than in leaves and stems, and the abiotic stimuli such as Seawater, NaCl, ABA, and PEG, could trigger a significant induction of SpFBA in S. portulacastrum roots within 2-12 h. Overproduction of Recombinant SpFBA resulted in an increased tolerance to salinity in transgenic Escherichia coli. All these results suggest that the SpFBA plays very important roles in responding to salt stress and related abiotic stimuli, and in improving the survival ability of S. portulacastrum under high salinity and drought.