Effect after introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton
Effect after introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton
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DOI：
10.1016/j.fcr.2003.11.001
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发表时间：
2004-05-10
影响因子：
5.8
通讯作者：
Wu, YK
中科院分区：
文献类型：
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作者：
Chen, DH;Ye, GY;Wu, YK
苏云金芽孢杆菌(Bt)转基因棉花表现出营养生长和生殖生长特性的变化。本研究的目的是探讨与 Bt 棉花品种生长密切相关的氮代谢的生理变化。该研究是在 2001 年和 2002 年生长季在中国扬州扬州大学农场对两个 131 个转基因棉花品种及其亲本进行的，其中一个是常规亲本（兴阳 822）和轮回亲本（苏棉 9 号），另一个是杂种（库棉 1 号）和母本（豫棉 1 号）。 2001 年的研究结果表明，Bt 棉花品种均高于其亲本 花铃发育高峰期，叶片全氮、游离氨基酸和可溶性蛋白含量，NR、GPT活性较高，蛋白酶活性较低。全氮增幅最大的是盛铃期，库棉一号和兴阳822分别增幅36.01%和18.96%。游离氨基酸和可溶性蛋白质含量也有类似的结果。 2002年的研究结果进一步表明，NR活性在盛花期和吐絮期显着增加，其中吐絮期增幅最大，库棉一号和兴烟822分别比亲本高出87.5%和61.4%；GPT活性在吐絮期增加最大，库棉一号和兴阳822分别比亲本高出39.1%和29.1%。分别是父母。而Bt品种的蛋白酶活性在花前和吐絮前期明显降低，其中花期前降低幅度最大，高峰期库棉Ⅰ号降低30%以上，兴阳822降低26.5%。此外，棉铃全氮含量急剧下降。结果表明，Bt棉品种叶片氮代谢强度高于亲本，特别是在方铃发育期。高氮条件下不利于方发育和铃早熟。栽培措施应以降低叶片氮代谢强度、保持营养生长和生殖生长的平衡为目标。 (C) 2003 Elsevier B.V. 保留所有权利。
Bacillus thuringiensis (Bt) transgenic cotton has shown changes of vegetative and reproductive growth characteristics. The objective of this study was to investigate the physiological change of nitrogen metabolism that related closely to the growth in Bt cotton cultivars. The study Was undertaken on two 131 transgenic cotton cultivars and their parents, one conventional (Xingyang822) and recurrent parent (Sumian No. 9), the other a hybrid (Kumian No. 1) and female parent (Yumian No. 1), during the 2001 and 2002 growing seasons at the Yangzhou University Farm, Yangzhou, China.In the 2001 study, The results indicated that the Bt cotton cultivars were higher than their parents in leaf total nitrogen, free amino acid and soluble protein content, greater in NR and GPT activity, and lower in protease activity, during peak square and boll developing period. The biggest increase of total nitrogen was at peak boll period, which increased by 36.01 and 18.96% for Kumian No. I and Xingyang822, respectively. There were similar results for free amino acid and soluble protein content. The results showed further in 2002 study that NR activity increased dramatically at peak square and early boll open period, the biggest increase at early boll open period, with Kumian No. I and Xingyan,822 being 87.5 and 61.4% higher than their parent, respectively, the biggest increase of GPT activity was at peak boll period, with Kumian No. I and Xingyang822 being 39.1 and 29.1% higher than their parent, respectively. However, protease activity of Bt cultivars reduced significantly before flowering and early boll open period, the biggest decrease was before flowering period, with Kumian No. I being more than 30%, Xingyang822 being 26.5% at peak square period. Moreover, the boll total nitrogen content reduced sharply.The results suggest that the Bt cotton cultivars have higher intensity of leaf nitrogen metabolism than their parent, especially during square and boll development period. It is disadvantage for square development and earlier boll maturity under high nitrogen condition. The cultural practice should aim at reducing leaf nitrogen metabolic strength and keep the balance of vegetative and reproductive growth. (C) 2003 Elsevier B.V. All rights reserved.