Major differences between WHV and HBV in the regulation of transcription
Major differences between WHV and HBV in the regulation of transcription
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DOI：
10.1006/viro.1996.8422
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发表时间：
1997-03-03
期刊：
影响因子：
3.7
通讯作者：
Mason, WS
中科院分区：
文献类型：
--
作者：
Di, Q;Summers, J;Mason, WS
进行了研究，以进一步表征土拨鼠肝炎病毒（WHV）基因组上的增强子和启动子元件。我们能够证实存在类似于相关的人甲型肝炎病毒（HBV）的主要启动子的WHV启动子和类似于最近描述的WHV E2元件的增强子（上田，K，Wei，Y.，Ganem，D.，Virology 217，413，1996）。然而，我们无法确定一个增强子类似的El元素（HBV），尽管事实上，这两种病毒共享高度的序列同源性和遗传组织。El区域的一些因子结合位点似乎在两种病毒之间是保守的，并且可能是WHV重叠X基因启动子活性所需的。其他人似乎不是必不可少的WHV X基因启动子活性，他们的功能活性，如果有的话，没有透露。我们未能在WHV区域中检测到与HBV E1增强子同源的功能性增强子元件，这可能表明（i）WHV和HBV的小环状基因组的转录调节存在根本差异;（ii）WHV含有F1元件，其在完整病毒基因组的背景下是功能性的，但在我们实验中使用的各种表达构建体的背景下不能发挥功能;或（iii）通过E1元件对WHV转录的正确调节依赖于在我们实验中使用的肝特异性细胞系中不表达的转录因子。(C)北京：科学出版社.
Studies were carried out to further characterize enhancer and promoter elements on the woodchuck hepatitis virus (WHV) genome. We were able to confirm the existence of WHV promoters analogous to the major promoters of the related human hepatitis a virus (HBV) and of an enhancer analogous to the recently described WHV E2 element (Ueda, K, Wei, Y., and Ganem, D., Virology 217, 413, 1996). However, we were unable to identify an enhancer analogous to the El element of (HBV), despite the fact that these two viruses share a high degree of sequence homology and genetic organization. Some factor binding sites in the El region appeared to be conserved between the two viruses and may be required for the activity of the overlapping X gene promoter of WHV. Others did not appear to be essential for WHV X gene promoter activity, and their functional activity, if any, was not revealed. Our failure to detect a functional enhancer element in the region of WHV homologous to the HBV El enhancer may indicate that (i) fundamental differences exist in transcriptional regulation of the small circular genomes of WHV and HBV; (ii) WHV contains an Fl element which is functional in the context of the intact viral genome, but which is unable to function in the context of the various expression constructs used in our experiments; or (iii) correct regulation of WHV transcription via an El element is dependent upon transcription factors which are not expressed in the liver-specific cell lines used in our experiments. (C) 1997 Academic Press.