The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility
The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility
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DOI：
10.1099/00221287-147-9-2517
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发表时间：
2001-09-01
期刊：
影响因子：
2.8
通讯作者：
Eberl, L
中科院分区：
文献类型：
--
作者：
Huber, B;Riedel, K;Eberl, L
洋葱伯克霍尔德菌和铜绿假单胞菌通常作为混合生物膜共存于患有囊性纤维化（CF）的患者的肺中。在此，分离了B的随机mini-Tn 5插入突变体。报道了洋葱H111在非生物表面上的生物膜形成中有缺陷。它表明，这些突变体之一不再产生N-酰基高丝氨酸内酯（AHLs）由于cepR基因的失活。cepR和cepI阿勒合酶基因共同构成了B的cep群体感应系统。洋葱。通过基因置换法，分别构建了cepI和cepR失活的两个确定的突变体H111-I和H111-R。这些突变体用于证明由B.洋葱H111需要一个功能性的cep群体感应系统。野生型和突变株形成的生物膜结构的详细定量分析表明，群体感应系统不参与初始细胞附着的调节，而是控制生物膜的成熟。此外，还证明了B.洋葱能够群集运动，这是一种被各种细菌利用的表面移位形式，以快速定殖于适当的基质。证明了B.洋葱是群体感应调节，可能通过控制生物表面活性剂的生产。用不同的生物表面活性剂补充cepR突变体H111-R可以恢复集群运动，而生物膜形成并未显着增加。这一结果表明，群集运动本身不是必不可少的生物膜形成的非生物表面。
Burkholderia cepacia and Pseudomonas aeruginosa often co-exist as mixed biofilms in the lungs of patients suffering from cystic fibrosis (CF). Here, the isolation of random mini-Tn5 insertion mutants of B. cepacia H111 defective in biofilm formation on an abiotic surface is reported. It is demonstrated that one of these mutants no longer produces N-acylhomoserine lactones (AHLs) due to an inactivation of the cepR gene. cepR and the cepI AHL synthase gene together constitute the cep quorum-sensing system of B. cepacia. By using a gene replacement method, two defined mutants, H111-I and H111-R, were constructed in which cepI and cepR, respectively, had been inactivated. These mutants were used to demonstrate that biofilm formation by B. cepacia H111 requires a functional cep quorum-sensing system. A detailed quantitative analysis of the biofilm structures formed by wild-type and mutant strains suggested that the quorum-sensing system is not involved in the regulation of initial cell attachment, but rather controls the maturation of the biofilm. Furthermore, it is shown that B. cepacia is capable of swarming motility, a form of surface translocation utilized by various bacteria to rapidly colonize appropriate substrata. Evidence is provided that swarming motility of B. cepacia is quorum-sensing-regulated, possibly through the control of biosurfactant production. Complementation of the cepR mutant H111-R with different biosurfactants restored swarming motility while biofilm formation was not significantly increased. This result suggests that swarming motility per se is not essential for biofilm formation on abiotic surfaces.