Comparison of polystyrene nanoparticles and UV-inactivated antigen-displaying adenovirus for vaccine delivery in mice.
Comparison of polystyrene nanoparticles and UV-inactivated antigen-displaying adenovirus for vaccine delivery in mice.
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DOI：
10.1186/1743-422x-10-108
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发表时间：
2013-04-05
期刊：
影响因子：
4.8
通讯作者：
Bayer W
中科院分区：
文献类型：
--
作者：
Johrden L;Tenbusch M;Lietz R;Bonsmann MS;Niezold T;Wildner O;Bayer W
惰性纳米粒子作为蛋白质疫苗的载体正引起人们的注意。在这里，我们评估的模型抗原卵清蛋白的免疫原性的聚苯乙烯颗粒上交付，并直接比较颗粒交付与腺病毒为基础的免疫。用可溶性卵清蛋白、卵清蛋白包被的不同大小的聚苯乙烯颗粒或编码并展示pIX-卵清蛋白融合蛋白的基于腺病毒的表达展示载体接种小鼠。与可溶性蛋白质给药相比，当卵清蛋白在聚苯乙烯颗粒上给药时，抗体应答明显更高，无论颗粒大小如何。与基于腺病毒的免疫相比，如果递送等量的蛋白质，则抗体水平较低，并且检测不到细胞免疫应答。我们在并排比较中证明，与用可溶性蛋白免疫相比，惰性纳米颗粒允许减少施用的抗原量，并诱导强烈增强的抗体应答，但与基于腺病毒的免疫相比，应答较低。
Inert nanoparticles are attracting attention as carriers for protein-based vaccines. Here we evaluate the immunogenicity of the model antigen ovalbumin delivered on polystyrene particles and directly compare particulate delivery with adenovirus-based immunization. Mice were vaccinated with soluble ovalbumin, ovalbumin-coated polystyrene particles of different sizes, or an adenovirus-based expression-display vector that encodes and displays a pIX-ovalbumin fusion protein. Antibody responses were clearly higher when ovalbumin was administered on polystyrene particles compared to soluble protein administration, regardless of the particle size. Compared to adenovirus-based immunization, antibody levels were lower if an equivalent amount of protein was delivered, and no cellular immune response was detectable. We demonstrate in a side-by-side comparison that inert nanoparticles allow for the reduction of the administered antigen amount compared to immunization with soluble protein and induce strongly enhanced antibody responses, but responses are lower compared to adenovirus-based immunization.