Design and mechanisms of antifouling materials for surface plasmon resonance sensors
Design and mechanisms of antifouling materials for surface plasmon resonance sensors
复制标题
表面等离子体共振传感器防污材料的设计与机理
DOI：
10.1016/j.actbio.2016.02.035
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发表时间：
2016-08-01
影响因子：
9.7
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Liu, Boshi;Liu, Xia;He, Zhimin
表面等离子体共振（SPR）生物传感器具有许多可能的应用，但受到传感器芯片表面结垢的限制，这阻碍了识别元件的固定和特异性结合。因此，迫切需要开发可折叠表面。本文首先讨论了自组装材料的形成机理，包括水化和空间位阻理论以及影响自组装效果的因素（分子结构和自组装单层结构、表面电荷、分子亲水性、接枝厚度和密度）。然后系统地综述了SPR生物传感器中应用于SPR生物传感器的接枝材料的最新进展，包括接枝策略、接枝能力及其优缺点。这些材料包括但不限于两性离子化合物、基于聚乙二醇的材料和基于多糖的材料。表面等离子体共振（SPR）是一种监测生物分子间相互作用的有力工具。SPR生物传感器的原理是将分子结合引起的折射率变化转化为共振光谱位移。然而，SPR金芯片表面的污垢是普遍存在的和麻烦的。SPR生物传感器的应用受到识别元件固定化和特异性结合的限制。因此，本文综述了SPR生物传感器的共振机理以及近年来在共振材料设计方面的研究进展，以期提高SPR生物传感器的准确性和灵敏度。据我们所知，这是第一次审查的重点是在SPR生物传感器的应用或有潜力的可再生材料。(C)2016 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Surface plasmon resonance (SPR) biosensors have many possible applications, but are limited by sensor chip surface fouling, which blocks immobilization and specific binding by the recognizer elements. Therefore, there is a pressing need for the development of antifouling surfaces. In this paper, the mechanisms of antifouling materials were firstly discussed, including both theories (hydration and steric hindrance) and factors influencing antifouling effects (molecular structures and self-assembled monolayer (SAM) architectures, surface charges, molecular hydrophilicity, and grafting thickness and density). Then, the most recent advances in antifouling materials applied on SPR biosensors were systematically reviewed, together with the grafting strategies, antifouling capacity, as well as their merits and demerits. These materials included, but not limited to, zwitterionic compounds, polyethylene glycol-based, and polysaccharide-based materials. Finally, the prospective research directions in the development of SPR antifouling materials were discussed.Statement of SignificanceSurface plasmon resonance (SPR) is a powerful tool in monitoring biomolecular interactions. The principle of SPR biosensors is the conversion of refractive index change caused by molecular binding into resonant spectral shifts. However, the fouling on the surface of SPR gold chips is ubiquitous and troublesome. It limits the application of SPR biosensors by blocking recognition element immobilization and specific binding. Hence, we write this paper to review the antifouling mechanisms and the recent advances of the design of antifouling materials that can improve the accuracy and sensitivity of SPR biosensors. To our knowledge, this is the first review focusing on the antifouling materials that were applied or had potential to be applied on SPR biosensors. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.