Determination of Carotenoids in Egg Yolk by High Performance Liquid Chromatography with Vortex-Assisted Hollow Fiber Liquid-Phase Microextraction using Mixed Extraction Solvent.
Determination of Carotenoids in Egg Yolk by High Performance Liquid Chromatography with Vortex-Assisted Hollow Fiber Liquid-Phase Microextraction using Mixed Extraction Solvent.
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DOI：
10.1093/chromsci/bmw130
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发表时间：
2016-10
影响因子：
1.3
通讯作者：
Juan Wang;N. Wu;Yaling Yang
中科院分区：
文献类型：
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作者：
Juan Wang;N. Wu;Yaling Yang
建立了以混合溶剂为萃取剂的涡旋辅助中空纤维液相微萃取-高效液相色谱二极管阵列检测法，用于蛋黄中叶黄素和β-胡萝卜素的提取和测定。该方法基于将样品溶液中的类胡萝卜素微萃取到位于中空纤维腔中的萃取剂（20 μL）中，然后涡旋混合。采用正辛醇+十一醇（6：4，v：v）的混合溶剂作为提取剂，通过中空纤维的方式提取具有适宜粘度且与类胡萝卜素相容的类胡萝卜素。利用涡旋混合提供样品溶液的有效和温和的混合，并增加分析物和中空纤维的边界层之间的接触。对影响回收率的参数进行了研究和优化。在最佳条件下，叶黄素和β-胡萝卜素的线性范围为50 ~ 1000 ng mL-1，相关系数>0.9982，相对标准偏差（n = 5）为2.23%~ 3.51%，检出限分别为0.038和0.045 μg mL-1。该方法用于蛋黄中类胡萝卜素的提取和测定，回收率为90.2-101.8%。
A vortex-assisted hollow fiber liquid-phase microextraction method using a mixed solvent as the extraction solvent followed by high performance liquid chromatography diode-array detection was developed for the extraction and determination of two carotenoids including lutein and β-carotene from egg yolk. The method is based on the microextraction of carotenoids from sample solution into extracting agent (20 μL), which is located in the lumen of hollow fiber followed by vortex-mixing. The mixed solvent (1-octanol+1-undecanol, 6:4, v:v) was employed as extracting agent for it has suitable viscosity and compatible with carotenoids via hollow fiber. Vortex-mixing was utilized to provide effective and mild mixing of sample solution and increase the contact between analytes and boundary layers of the hollow fiber. Parameters influencing recoveries were investigated and optimized. Under the optimum conditions, the linear range of lutein and β-carotene were from 50 to 1,000 ng mL-1 The correlation coefficients of the calibration curves were >0.9982, relative standard deviations (n = 5) were between 2.23% and 3.51% and the limits of detection were 0.038 and 0.045 μg mL-1 for lutein and β-carotene, respectively. The proposed method was successfully applied to the extraction and determination of caroteonids in egg yolk with the satisfactory relative recoveries (90.2-101.8%).