Comparative phenotype of circulating versus tissue immune cells in human lung and blood compartments during health and disease.
Comparative phenotype of circulating versus tissue immune cells in human lung and blood compartments during health and disease.
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DOI：
10.1093/discim/kyad009
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发表时间：
2023
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肺是一个动态的粘膜表面，不断暴露于各种免疫学挑战，包括无害的环境抗原，污染物和潜在的侵入性微生物。免疫系统在这一关键部位的失调与一系列慢性炎症性疾病有关，包括哮喘和慢性肺阻塞性疾病（COPD）。然而，由于其相对难以接近，我们对人肺免疫区室的基本理解是有限的。为了解决这个问题，我们对人肺组织进行了流式细胞术免疫表型分析，并匹配了从115名接受肺组织切除术的供体中分离的血液样本。我们提供了肺单核吞噬细胞和T细胞区室的详细特征，证明了肺组织细胞与外周循环细胞之间存在明显的表型差异。此外，我们表明，CD 103表达区分肺T细胞，最近经历了TCR和IL-7 R信号。出乎意料的是，我们发现哮喘或COPD供体的免疫景观与对照组大致相当。我们的数据为我们对健康和疾病中肺部免疫区室的理解提供了急需的扩展。
The lung is a dynamic mucosal surface constantly exposed to a variety of immunological challenges including harmless environmental antigens, pollutants, and potentially invasive microorganisms. Dysregulation of the immune system at this crucial site is associated with a range of chronic inflammatory conditions including asthma and Chronic Pulmonary Obstructive Disease (COPD). However, due to its relative inaccessibility, our fundamental understanding of the human lung immune compartment is limited. To address this, we performed flow cytometric immune phenotyping of human lung tissue and matched blood samples that were isolated from 115 donors undergoing lung tissue resection. We provide detailed characterization of the lung mononuclear phagocyte and T cell compartments, demonstrating clear phenotypic differences between lung tissue cells and those in peripheral circulation. Additionally, we show that CD103 expression demarcates pulmonary T cells that have undergone recent TCR and IL-7R signalling. Unexpectedly, we discovered that the immune landscape from asthmatic or COPD donors was broadly comparable to controls. Our data provide a much-needed expansion of our understanding of the pulmonary immune compartment in both health and disease.