TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast
TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast
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DOI：
10.1016/j.bbrc.2019.11.064
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发表时间：
2020-01-29
影响因子：
3.1
通讯作者：
Ushimaru, Takashi
中科院分区：
文献类型：
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作者：
Morshed, Shamsul;Sharmin, Tasnuva;Ushimaru, Takashi
微自噬在营养饥饿和雷帕霉素复合物1（TORC 1）激酶靶点失活后促进。微自噬需要通过转运所需的内体分选复合物（ESCRT）对液泡膜进行内陷。Vps 27是ESCRT-0的亚基，在TORC 1失活后通过去磷酸化被募集到液泡膜上。在这里，我们发现Hsel，另一个ESCRT-0亚基，也被招募到液泡膜后TORC 1失活，促进ESCRT-0复合物的形成液泡膜。Hsel募集依赖于Vps 27，而Vps 27募集独立于Hsel。不仅Vps 27，而且Hsel需要ESCRT-III招聘到空泡膜和TORC 1失活后的微自噬诱导。这项研究表明，ESCRT-0（Vps 27-Hsel）复合物的液泡膜上的形成是重要的TORC 1灭活后的微自噬失活。(C)2019爱思唯尔公司All rights reserved.
Microautophagy is promoted after nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) kinase. Invagination of vacuolar membranes by endosomal sorting complex required for transport (ESCRT) is required for microautophagy. Vps27, a subunit of ESCRT-0, is recruited onto vacuolar membranes via dephosphorylation after TORC1 inactivation. Here, we showed that Hsel, another ESCRT-0 subunit, is also recruited onto vacuolar membranes after TORC1 inactivation, promoting formation of ESCRT-0 complex on vacuolar membranes. Hsel recruitment was dependent on Vps27, whereas Vps27 recruitment was independent of Hsel. Not only Vps27 but also Hsel was required for ESCRT-III recruitment onto vacuolar membranes and microautophagy induction after TORC1 inactivation. This study revealed that ESCRT-0 (Vps27-Hsel) complex formation on vacuolar membranes is important for microautophagy inactivation after TORC1 inactivation. (C) 2019 Elsevier Inc. All rights reserved.