Co-option of the PRDM14-CBFA2T complex from motor neurons to pluripotent cells during vertebrate evolution
Co-option of the PRDM14-CBFA2T complex from motor neurons to pluripotent cells during vertebrate evolution
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DOI：
10.1242/dev.168633
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发表时间：
2019-01
期刊：
影响因子：
4.6
通讯作者：
Masanori Kawaguchi;K. Sugiyama;K. Matsubara;Che-Yi Lin;Shigehiro Kuraku;Shota Hashimoto;Yoshiaki Suwa;Luok Wen Yong;Koji Takino;Shota Higashida;Daisuke Kawamura;Jr-Kai Yu;Y. Seki
中科院分区：
文献类型：
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作者：
Masanori Kawaguchi;K. Sugiyama;K. Matsubara;Che-Yi Lin;Shigehiro Kuraku;Shota Hashimoto;Yoshiaki Suwa;Luok Wen Yong;Koji Takino;Shota Higashida;Daisuke Kawamura;Jr-Kai Yu;Y. Seki
摘要在哺乳动物中，包括Pou5f1在内的转录因子的核心回路控制着细胞多能性的基因调控网络。然而，后口动物中与多能性相关的转录网络的进化起源和转化还没有被阐明。PR结构域包含蛋白14(PRDM14)在多能细胞和生殖细胞中特异表达，是建立小鼠胚胎干细胞和原始生殖细胞所必需的。在这里，我们比较了PRDM14同源基因在后口动物中的功能和表达模式。文昌鱼PRDM14和斑马鱼PRDM14，而不是海胆PRDM14，补偿了小鼠PRDM14维持小鼠胚胎干细胞多能性的功能。有趣的是，海胆PRDM14和海胆CBFA2T是PRDM14在小鼠ESCs中的重要合作伙伴，它们补充了小鼠PRDM14 KO ESCs的自我更新缺陷。与PRDM14在小鼠胚胎中的表达模式相反，PRDM14在文昌鱼胚胎的运动神经元中表达，就像在斑马鱼胚胎中观察到的那样。因此，PRDM14在运动神经元中的表达在非四足后口动物中是保守的，并且PRDM14-CBFA2T复合体从运动神经元到多能细胞的共同选择可能在脊椎动物进化过程中维持了多能性的转录网络。本文有一个与之相关的《报纸背后的人》采访。重点文章：对PRDM14同源基因在后口动物中的功能和表达模式的比较揭示了一个解决多潜能转录网络如何进化的框架。
ABSTRACT Gene regulatory networks underlying cellular pluripotency are controlled by a core circuitry of transcription factors in mammals, including POU5F1. However, the evolutionary origin and transformation of pluripotency-related transcriptional networks have not been elucidated in deuterostomes. PR domain-containing protein 14 (PRDM14) is specifically expressed in pluripotent cells and germ cells, and is required for establishing embryonic stem cells (ESCs) and primordial germ cells in mice. Here, we compared the functions and expression patterns of PRDM14 orthologues within deuterostomes. Amphioxus PRDM14 and zebrafish PRDM14, but not sea urchin PRDM14, compensated for mouse PRDM14 function in maintaining mouse ESC pluripotency. Interestingly, sea urchin PRDM14 together with sea urchin CBFA2T, an essential partner of PRDM14 in mouse ESCs, complemented the self-renewal defect in mouse Prdm14 KO ESCs. Contrary to the Prdm14 expression pattern in mouse embryos, Prdm14 was expressed in motor neurons of amphioxus embryos, as observed in zebrafish embryos. Thus, Prdm14 expression in motor neurons was conserved in non-tetrapod deuterostomes and the co-option of the PRDM14-CBFA2T complex from motor neurons into pluripotent cells may have maintained the transcriptional network for pluripotency during vertebrate evolution. This article has an associated ‘The people behind the papers’ interview. Highlighted Article: A comparison of the functions and expression patterns of PRDM14 orthologues within deuterostomes reveals a framework for addressing how pluripotency transcriptional networks have evolved.