Siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads
Siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads
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DOI：
10.1128/aem.68.6.2745-2753.2002
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发表时间：
2002-06-01
影响因子：
4.4
通讯作者：
Palleroni, NJ
中科院分区：
文献类型：
--
作者：
Meyer, JM;Geoffroy, VA;Palleroni, NJ
对301株荧光假单胞菌进行了铁质分型分析，即通过对其主要铁载体和吡喃类化合物的等电泳谱分析，以及对吡喃类化合物介导的铁摄取专一性的测定，分析了301株以前通过传统表型和/或基因组分类方法鉴定的荧光假单胞菌。一般说来，一个分类群内的菌株，即油菜假单胞菌、烟曲假单胞菌、杰森假单胞菌、曼德利假单胞菌、孟特利假单胞菌、“莫赛氏假单胞菌”、“帕氏假单胞菌”、“罗氏假单胞菌”、“沙门氏假单胞菌”、“丁香假单胞菌”、“三维瓦氏假单胞菌”、“托拉西氏假单胞菌”、“维氏假单胞菌”和基因种FP 1、FP 2和FP 3产生相同的绿脓杆菌，这是该组的特征，因为它在结构上与其他组产生的红假单胞菌不同。相比之下，28株属于臭名昭著的异质性荧光假单胞菌的菌株的特征是在吡哆醇水平上具有很大的异质性。对23个部分特征的表型群的研究表明，铁质分型在暗示群和定义明确的物种之间的相关性以及检测错误分类的单个菌株方面非常有用，DNA-DNA杂交证实了这一点。铁质分型作为一种确定工具的用处被扩展到非荧光物种波纹假单胞菌、弗雷德伯根假单胞菌、禾谷假单胞菌和长舌假单胞菌，这些物种被认为具有相同的物种特异性铁载体系统，因此很容易相互区分。因此，快速、准确和易于操作的铁质分型方法比目前在物种水平上准确鉴定假单胞菌所需的通常的表型和基因组方法更有利。
A total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. As a general rule, strains within a well-circumscribed taxonomic group, namely the species Pseudomonas brassicacearum, Pseudomonas fuscovaginae, Pseudomonas jessenii, Pseudomonas mandelii, Pseudomonas monteilii, "Pseudomonas mosselii," Pseudomonas palleronii," Pseudomonas rhodesiae, "Pseudomonas salomonii," Pseudomonas syringae, Pseudomonas thivervalensis, Pseudomonas tolaasii, and Pseudomonas veronii and the genomospecies FP1, FP2, and FP3 produced an identical pyoverdine which, in addition, was characteristic of the group, since it was structurally different from the pyoverdines produced by the other groups. In contrast, 28 strains belonging to the notoriously heterogeneous Pseudomonas fluorescens species were characterized by great heterogeneity at the pyoverdine level. The study of 23 partially characterized phenotypic clusters demonstrated that siderotyping is very useful in suggesting correlations between clusters and well-defined species and in detecting misclassified individual strains, as verified by DNA-DNA hybridization. The usefulness of siderotyping as a determinative tool was extended to the nonfluorescent species Pseudomonas corrugata, Pseudomonas frederiksbergensis, Pseudomonas graminis, and Pseudomonas plecoglossicida, which were seen to have an identical species-specific siderophore system and thus were easily differentiated from one another. Thus, the fast, accurate, and easy-to-perform siderotyping method compares favorably with the usual phenotypic and genomic methods presently necessary for accurate identification of pseudomonads at the species level.