Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis
Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis
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Rubisco积累对于拟南芥fln2-4突变体的绿化很重要
DOI：
10.1016/j.plantsci.2015.04.005
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发表时间：
2015-07-01
期刊：
影响因子：
5.2
通讯作者：
Yang, Zhong-Nan
中科院分区：
文献类型：
--
作者：
Huang, Chao;Yu, Qing-Bo;Yang, Zhong-Nan
果糖激酶样蛋白 2 (FLN2) 是 PEP 复合物的组成部分。 FLN2 敲除突变体在含蔗糖的培养基上表现出延迟绿化表型。我们之前的工作表明，部分 PEP 活性对其绿化表型至关重要。在这项研究中，我们进一步报道了足够的 Rubisco 积累对于 fln2-4 绿化至关重要。糖具有许多重要的功能，例如能量来源和信号分子。通过使用糖类似物和糖信号抑制剂的药理学实验，我们证明糖可以作为支持 fln2-4 绿化的能量。种子储备和光合作用二氧化碳固定是幼苗早期生长的主要能源。通过比较野生型和fln2-4的种子大小和黑暗发芽情况，没有观察到野生型和fln2-4的种子储备存在明显差异，这表明缺陷的碳固定可能是fln2-4在幼苗生长早期能量不足的原因。 fln2-4中Rubisco含量较低，但在fln2-4绿化过程中迅速积累。核编码 rbcL 基因的表达促进 Rubisco 积累并部分补充突变缺陷。这些结果表明 Rubisco 积累对于 fln2-4 绿化至关重要。总之，依赖于足够的 PEP 活性的 Rubisco 快速积累对于正常的幼苗生长非常重要。 (C) 2015 Elsevier Ireland Ltd. 保留所有权利。
The fructokinase-like protein2 (FLN2) is a component of the PEP complex. FLN2 knockout mutants displayed a delayed greening phenotype on sucrose-containing medium. Our previous work indicated that partial PEP activity is essential for its greening phenotype. In this study, we further report that sufficient Rubisco accumulation is critical for fln2-4 greening. Sugar serves many important functions, such as an energy source and signaling molecule. Through pharmacological experiments using a sugar analog and sugar signaling inhibitor, we demonstrate that sugar serves as energy to support the fln2-4 greening. Seed-reserve and photosynthetic CO2-fixation are the primary energy sources for early seedling growth. No obvious differences were observed in the seed-reserve of the wild-type and fln2-4 by comparing their seed size and dark-germination, indicating that the defective carbon fixation may account for the energy deficit in fln2-4 during its early seedling growth. The Rubisco content was low in fln2-4, but it rapidly accumulated during the greening of fln2-4. Expression of a nuclear-encoded rbcL gene facilitates Rubisco accumulation and partially complements the mutant defects. These results suggest that the Rubisco accumulation is critical for fln2-4 greening. In summary, the rapid Rubisco accumulation that depends on sufficient PEP activity is important for normal seedling growth. (C) 2015 Elsevier Ireland Ltd. All rights reserved.