A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents.
A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents.
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DOI：
10.1016/j.jneumeth.2023.109827
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发表时间：
2023-04-15
影响因子：
3
通讯作者：
Duguid, Ian
中科院分区：
文献类型：
--
作者：
Dacre, Joshua;Rivera, Michelle Sanchez;Schiemann, Julia J.;Currie, Stephen;Ammer, Julian J.;Duguid, Ian
在体内膜片钳记录技术提供了访问的亚和阈上膜电位动态的个别神经元在行为。然而，在整个行为过程中保持记录稳定性是一个重大挑战，虽然头部保护的方法通常用于增强稳定性，但与颅骨相关的行为相关的大脑运动可能严重影响全细胞膜片钳记录的成功率和持续时间。我们开发了一种低成本、生物相容性和3D打印的颅骨植入物，能够局部稳定大脑运动，同时与传统开颅术相比，允许进入大脑。在头部受限行为小鼠中的实验表明，颅骨植入物可以可靠地降低大脑位移的幅度和速度，显著提高重复运动行为记录的成功率。我们的解决方案提供了对目前可用的大脑稳定策略的改进。由于其小尺寸，植入物可以改装为大多数体内电生理记录设置，提供了一种低成本，易于实施的解决方案，用于增加体内细胞内记录的稳定性。通过促进体内稳定的全细胞膜片钳记录，生物相容性3D打印植入物应该会加速对单神经元计算基础行为的研究。用于稳定在体膜片钳记录的颅骨植入物。低成本、易于实施的解决方案，以提高记录稳定性。3-D可打印的生物相容性植入物，可以改装为任何制剂。增加体内膜片钳记录的成功率和寿命。
In vivo patch-clamp recording techniques provide access to the sub- and suprathreshold membrane potential dynamics of individual neurons during behavior. However, maintaining recording stability throughout behavior is a significant challenge, and while methods for head restraint are commonly used to enhance stability, behaviorally related brain movement relative to the skull can severely impact the success rate and duration of whole-cell patch-clamp recordings. We developed a low-cost, biocompatible, and 3D-printable cranial implant capable of locally stabilizing brain movement, while permitting equivalent access to the brain when compared to a conventional craniotomy. Experiments in head-restrained behaving mice demonstrate that the cranial implant can reliably reduce the amplitude and speed of brain displacements, significantly improving the success rate of recordings across repeated bouts of motor behavior. Our solution offers an improvement on currently available strategies for brain stabilization. Due to its small size, the implant can be retrofitted to most in vivo electrophysiology recording setups, providing a low cost, easily implementable solution for increasing intracellular recording stability in vivo. By facilitating stable whole-cell patch-clamp recordings in vivo, biocompatible 3D printed implants should accelerate the investigation of single neuron computations underlying behavior. Cranial implant for stabilizing patch-clamp recordings in vivo. Low cost, easily implementable solution to improve recording stability. 3-D printable, biocompatible implant that can be retrofitted to any preparation. Increases the success rate and longevity of in vivo patch-clamp recordings.
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影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
小脑-丘脑皮质通路驱动行为环境相关的运动启动。
影响因子：
16.2
作者：
Dacre J;Colligan M;Clarke T;Ammer JJ;Schiemann J;Chamosa-Pino V;Claudi F;Harston JA;Eleftheriou C;Pakan JMP;Huang CC;Hantman AW;Rochefort NL;Duguid I
通讯作者：
Duguid I
影响因子：
3
作者：
Greenberg, David S.;Kerr, Jason N. D.
通讯作者：
Kerr, Jason N. D.
影响因子：
64.8
作者：
NEHER, E;SAKMANN, B
通讯作者：
SAKMANN, B
影响因子：
5.5
作者：
Chen, Jerry L.;Pfaeffli, Oliver A.;Helmchen, Fritjof
通讯作者：
Helmchen, Fritjof