Bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus Phanerochaete sordida
Bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus Phanerochaete sordida
复制标题
DOI：
10.1016/j.jhazmat.2016.09.049
复制
发表时间：
2017-01-05
影响因子：
13.6
通讯作者：
Hirai, Hirofumi
中科院分区：
文献类型：
--
作者：
Mori, Toshio;Wang, Jianqiao;Hirai, Hirofumi
噻虫胺（Clothianidin，CLO）是一种在世界范围内广泛使用的烟碱类杀虫剂。然而，其对蜜蜂的毒性和对人类的神经毒性是亟待解决的问题。在氮限制的液体培养基中，研究了白腐真菌花脸革菌对CLO的降解。在30 ℃下孵育20天后，37%的CLO在培养物中降解。对培养物上清液的高分辨率ESI-MS和NMR分析鉴定出N-（2-氯噻唑-5-基-甲基）-N '-甲基脲（TZMU）是CLO降解的代谢产物。在培养基中添加细胞色素P450抑制剂可显著降低花脸青霉对CLO的降解。并且该真菌分泌的主要木质素降解酶锰过氧化物酶不对CLO进行降解。CLO和TZMU对神经元细胞系Neuro2a的活力的影响表明，臭皮霉有效地将CLO降解成缺乏神经毒性的代谢物。(C)2016爱思唯尔B.V.保留所有权利。
Clothianidin (CLO) is a member of the neonicotinoid pesticides, which have been widely used worldwide over the last two decades. However, its toxicity for bees and neurological toxicity for humans are urgent problems. Here, the degradation of CLO by the white-rot fungus Phanerochaete sordida was examined in nitrogen-limited liquid medium. After incubation for 20 days at 30 degrees C, 37% of CLO was degraded in the cultures. High-resolution ESI-MS and NMR analyses of the culture supernatant identified N-(2-chlorothiazol-5-yl-methyl)-N'-methylurea (TZMU) as a metabolite of CLO degradation. The addition of cytochrome P450 inhibitors to the culture medium markedly reduced the degradation of CLO by P. sordida. And manganese peroxidase, a major ligninolytic enzyme secreted by this fungus, were not carried out CLO degradation. The effects of CLO and TZMU on the viability of the neuronal cell line Neuro2a demonstrated that P. sordida effectively degrades CLO into a metabolite that lacks neurotoxicity. (C) 2016 Elsevier B.V. All rights reserved.