RHEUMATOID FACTOR-LIKE REACTANTS IN SERA PROVEN TO TRANSMIT NON-A, NON-B HEPATITIS - A POTENTIAL SOURCE OF FALSE-POSITIVE REACTIONS IN NON-A, NON-B ASSAYS
RHEUMATOID FACTOR-LIKE REACTANTS IN SERA PROVEN TO TRANSMIT NON-A, NON-B HEPATITIS - A POTENTIAL SOURCE OF FALSE-POSITIVE REACTIONS IN NON-A, NON-B ASSAYS
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DOI：
10.1002/hep.1840050204
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发表时间：
1985-01-01
期刊：
影响因子：
13.5
通讯作者：
PURCELL, RH
中科院分区：
文献类型：
--
作者：
SHIRAISHI, H;ALTER, HJ;PURCELL, RH
在固相放射免疫测定（RIA）中利用恢复期非A、非B（NANB）人和黑猩猩血清，试图鉴定人血浆、血浆衍生的颗粒和NP-40破坏的颗粒中的特异性NANB抗原，所述颗粒被证明将NANB感染传播给黑猩猩。RIA反应在8个家系NANB感染性血浆中的5个中观察到，但似乎不是病毒特异性的。RIA反应分数：在CsCl中的密度为1.27-1.32 g/cm 3;显示对应于正常IgM的免疫反应性，但峰值RIA活性与异常IgM一致，其峰值沉降系数为56 S [沉降系数]，并且在比正常IgM更高的pH（5.6-5.0）下在色谱聚焦柱上洗脱。该反应物与类风湿因子共有的性质是它被聚集的IgG吸收，对2-巯基乙醇不稳定，但在类风湿因子的标准凝集试验中不反应。在黑猩猩和人的NANB感染的急性和慢性阶段都检测到该反应物。通过与假定的NANB恢复期血清中的IgG反应，这种异常的类风湿因子可以模拟NANB抗原，并在假定的NANB检测中代表假阳性反应的原因。
Convalescent phase non-A, non-B (NANB) human and chimpanzee sera were utilized in a solid-phase radioimmunoassay (RIA) in an attempt to identify a specific NANB antigen in human plasma, plasma-derived pellets and NP-40 disrupted pellets proven to transmit NANB infection to chimpanzees. RIA reactivity was noted in 5 of 8 pedigreed NANB infectious plasmas, but did not appear to be virus-specific. The RIA reactive fraction: had a density of 1.27-1.32 g/cm3 in CsCl; showed immunoreactivity corresponding to normal IgM, but peak RIA activity coincided with an aberrant IgM having a peak sedimentation coefficient of 56 S [sedimentation coefficient] and eluted at higher pH (5.6-5.0) than normal IgM on chromatofocusing columns. The reactant shared properties with rheumatoid factor in that it was absorbed by aggregated IgG and was unstable to 2-mercaptoethanol, but it did not react in standard agglutination tests for rheumatoid factor. The reactant was detected in both the acute and chronic phase of NANB infections in chimpanzee and man. By reacting with IgG in presumptive NANB convalescent sera, this aberrant rheumatoid factor could simulate a NANB antigen and represent a cause of false-positive reactions in putative NANB assays.