Mutations that affect the translation efficiency of Tn9-derived cat gene in Bacillus subtilis.
Mutations that affect the translation efficiency of Tn9-derived cat gene in Bacillus subtilis.
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影响枯草芽孢杆菌 Tn9 衍生 cat 基因翻译效率的突变。
DOI：
10.1073/pnas.82.1.173
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发表时间：
1985
影响因子：
11.1
通讯作者：
Rodriguez,RL
中科院分区：
文献类型：
--
作者：
Lin,CK;Goldfarb,DS;Doi,RH;Rodriguez,RL
我们已经分离出两种自发突变，其增加枯草芽孢杆菌中Tn 9衍生的cat基因的表达。这些突变似乎影响氯霉素乙酰转移酶（CAT;乙酰辅酶A：氯霉素3-O-乙酰转移酶，EC 2.3.1.28）翻译的起始，由位于cat 5'端的55个碱基对序列的串联重复和三重化组成。重复序列中包括Shine-Dalgarno位点、起始密码子和位于cat基因结构部分内的二联体对称区域。突变的起始位点的一个显著特征是它们在猫信使RNA的5'端形成茎环结构的潜力。在这些结构的单链环内是cat基因的核糖体结合位点和起始密码子。似乎革兰氏阴性猫翻译起始位点已经突变，以允许在B中有效利用。枯草芽孢杆菌，而不直接影响Shine-Dalgarno序列同源性。这一报告表明，二级结构附近的Shine-Dalgarno网站可以发挥强大的积极影响的翻译开始在B。枯草芽孢杆菌
We have isolated two spontaneous mutations that increase the expression of the Tn9-derived cat gene in Bacillus subtilis. These mutations, which appear to affect initiation of translation of chloramphenicol acetyltransferase (CAT; acetyl-CoA:chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) consist of a tandem duplication and triplication of a 55-base-pair sequence located at the 5' end of cat. Included in the repeated sequence are the Shine-Dalgarno site, initiation codon, and a region of dyad symmetry located within the structural portion of the cat gene. A striking feature of the mutated initiation sites is their potential to form stem-loop structures at the 5' end of the cat messenger RNA. Within the single-stranded loops of these structures are the ribosome binding site and initiation codon for the cat gene. It appears that the Gram-negative cat translation initiation site has mutated to permit efficient utilization in B. subtilis without directly affecting Shine-Dalgarno sequence homology. This report suggests that secondary structure in the vicinity of the Shine-Dalgarno site can exert a strong positive influence on the initiation of translation in B. subtilis.