Allergen-induced histamine release in intact human skin in vivo assessed by skin microdialysis technique: Characterization of factors influencing histamine releasability
Allergen-induced histamine release in intact human skin in vivo assessed by skin microdialysis technique: Characterization of factors influencing histamine releasability
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DOI：
10.1016/s0091-6749(96)70313-5
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发表时间：
1996-02-01
影响因子：
14.2
通讯作者：
Skov, PS
中科院分区：
文献类型：
--
作者：
Petersen, LJ;Mosbech, H;Skov, PS
背景资料：该研究的目的是通过使用一种新的微透析技术，并研究协变量影响组胺releasables.Methods：中空微透析纤维插入到15个Timothy敏感的主题在真皮上层的特点过敏原诱导的组胺释放在人体皮肤中。每名受试者最多插入12根纤维。以3.0 μ l/min的速率用Krebs-Ringer溶液灌注每根纤维。通过皮内注射或皮肤点刺试验将三至四种过敏原系列稀释液应用于皮肤。在皮肤激发前分两次（2分钟）收集样本，并在皮肤激发后连续收集10份样本（20分钟）。结果：皮内试验和皮肤点刺试验均显示组胺释放呈明显的剂量-反应关系。皮内试验后组胺释放达到峰值的时间为4 ~ 8分钟，而皮肤点刺试验为12 ~ 14分钟。组胺释放与风团大小显著相关。组胺释放的受试者内变异系数约为20%。观察到组胺释放性的显著受试者间差异。70%至75%的变化可以解释由性别，总的和过敏原特异性IgE的组合，并在体外嗜碱性粒细胞组胺释放test.Conclusion：使用皮肤微透析技术，我们已经详细描述了组胺释放在完整的人体皮肤过敏原。微透析方法被证明是一种可重复的技术，监测组胺释放过敏性皮肤反应和研究组胺释放的皮肤肥大细胞在体内。
Background: The purposes of the study were to characterize allergen-induced histamine release in intact human skin in vivo by using a novel microdialysis technique and to study covariates influencing histamine releasability.Methods: Hollow microdialysis fibers were inserted in to the upper dermis in 15 timothy-sensitive subjects. Up to 12 fibers were inserted in each subject. Each fiber was perfused with Krebs-Ringer's solution at a rate of 3.0 mu l/min. Three to four serial dilutions of allergen were applied to the skin by intracutaneous injections or skin prick test above individual fibers. Samples were collected in two 2-minute fractions before skin challenge and in 10 consecutive samples for 20 minutes after skin challenge. Histamine was assayed spectrofluorometrically.Results: A significant dose-response relationship for histamine release was demonstrated with intracutaneous tests and skin prick tests. The time to reach peak histamine release after an intracutaneous test was 4 to 8 minutes, compared with 12 to 14 minutes for a skin prick test. Histamine release correlated significantly with wheal size. Intrasubject coefficient of variation on histamine release was about 20%. A substantial intersubject variation in histamine releasability was observed. Seventy to seventy-five percent of the variation could be accounted for by a combination of gender, total and allergen-specific IgE, and an in vitro basophil histamine release test.Conclusion: Using a skin microdialysis technique, we have described in detail histamine release in intact human skin by allergen. The microdialysis method proved to be a reproducible technique for monitoring histamine release in allergic skin reactions and for studying histamine releasability of skin mast cells in vivo.