Sequential passage of influenza virus in embryonated eggs or tissue culture: emergence of mutants.
Sequential passage of influenza virus in embryonated eggs or tissue culture: emergence of mutants.
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流感病毒在含胚卵或组织培养物中的连续传代：突变体的出现。
DOI：
10.1016/0042-6822(80)90309-8
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Palese,P
中科院分区：
文献类型：
--
作者：
Brand,C;Palese,P
通过 A/WSN/33 病毒在胚蛋、MDBK 细胞和 MDCK 细胞中的传代来检查流感病毒突变体的出现。从噬斑纯化的 A/WSN/33 病毒制剂中，通过 MDCK 细胞中 12 次噬斑到噬斑的传代衍生出四个独立的克隆。 A/WSN/33 病毒在 MDBK 细胞中进行 8-12 次空斑到空斑传代后，分离出 4 个克隆，并在有限稀释的含胚卵中通过 12 次传代衍生出 1 个克隆。与噬斑纯化的 A/WSN/33 病毒的原始制剂的图谱相比，由这些传代独立衍生的所有九个克隆在寡核苷酸图谱上都显示出差异。相比之下，低稀释度（每次接种10'-10' PFU）的含胚卵中的12次传代产生了A/WSN/33病毒群体，通过寡核苷酸作图无法将其与原始制剂区分开来。甲型流感病毒变异体在空斑到空斑传代过程中频繁出现，这与以下发现形成鲜明对比：通过寡核苷酸作图比较，四分之三的水泡性口炎病毒克隆（源自 MDCK 细胞中独立的空斑到空斑传代）与原始制剂相同。
The emergence of influenza virus mutants was examined by passage of A/WSN/33 virus in embryonated eggs, in MDBK cells, and in MDCK cells. From a plaque-purified A/WSN/33 virus preparation, four independent clones were derived by 12 plaque-to-plaque passages in MDCK cells. Four clones were isolated following 8–12 plaque-to-plaque passages of A/WSN/33 virus in MDBK cells, and one clone was derived by 12 passages in embryonated eggs at limiting dilution. All nine clones independently derived by these passages showed differences in their oligonucleotide maps when compared to the map of the original preparation of plaque-purified A/WSN/33 virus. In contrast, 12 passages in embryonated eggs at low dilutions (10'-10' PFU per inoculum) resulted in an A/WSN/33 virus population which was indistinguishable by oligonucleotide mapping from the original preparation. The frequent emergence of influenza A virus variants upon plaque-to-plaque passage contrasts with the finding that three out of four vesicular stomatitis virus clones, which were derived from independent plaque-to-plaque passage in MDCK cells, were identical to the original preparation when compared by oligonucleotide mapping.