The inflammation in the gut after experimental subarachnoid hemorrhage
The inflammation in the gut after experimental subarachnoid hemorrhage
复制标题
DOI：
10.1016/j.jss.2006.06.023
复制
发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Shi, Ji-xin
中科院分区：
文献类型：
--
作者：
Zhou, Meng-liang;Zhu, Lin;Shi, Ji-xin
背景SAH患者常伴有胃肠功能障碍。本研究验证了实验性SAH可诱导组织病理学变化的假设，即与肠道中NF-κ B活化途径相关的炎症反应。17只家兔随机分为对照组（n = 8）和SAH组（n = 9）。SAH组按“二次出血法”建立实验性SAH模型。采用组织病理学方法观察肠黏膜形态学变化，免疫组化方法检测TNF-α和ICAM-1的表达。采用电泳迁移率变动分析法测定NF-κ B结合活性。结果表明，SAH后肠粘膜出现一定程度的损伤性改变和白细胞浸润。SAH组TNF-α、ICAM-1阳性细胞数明显增多。SAH组肠组织NF-κ B B结合活性明显升高（P < 0.01）。本研究结果提示，兔SAH可诱导肠道NF-κ B和促炎细胞因子活化，并与形态学改变相关。(c)2007年爱思唯尔公司All rights reserved.
Background. Gastrointestinal dysfunction could be frequently observed in the patients suffering from SAH. This study test the hypothesis that experimental SAH could induce histopathological changes an inflammatory response associating with NF-kappa B activation pathway in the gut.Materials and methods. A total of 17 rabbits were randomly divided into two groups: control group (n = 8) and SAH group (n = 9). In the SAH group, the animals were subjected to experimental SAH according to the "two-hemorrhage" method. The histopathological study was performed to detect the intestinal mucosal morphological changes and immunohistochemical study was used to detect the TNF-alpha and ICAM-1 expressions. NF-kappa B binding activity was measured using the electrophoretic mobility shift assay.Results. It was demonstrated that some damage changes and leukocytes infiltration occurred in the intestinal mucosa after SAH. More positive cells for TNF-a and ICAM-1 were observed in the SAH group. The NF-kappa B binding activity in the intestines was significantly increased in the SAH group (P < 0.01).Conclusions. The results of the present study suggest that SAH in the rabbits could induce NF-kappa B and proinflammatory cytokines activation in the intestine, which is associated with morphological changes. (c) 2007 Elsevier Inc. All rights reserved.