High efficiency gene transfer into mammalian cells.
High efficiency gene transfer into mammalian cells.
复制标题
高效地将基因转移到哺乳动物细胞中。
DOI：
10.1098/rstb.1984.0137
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Peter W.J. Rigby
中科院分区：
文献类型：
--
作者：
Cornelia M. Gorman;David P. Lane;Peter W.J. Rigby
我们已经推广了基因转移的方法，大大增加了可用作外源基因受体的细胞的种类。我们的方法是使用一种瞬时测定系统，可以快速筛选外源DNA的表达。当基因转移的初始步骤已经用瞬时系统优化时，这些定义的条件用于产生有效的稳定转化。我们已经看到，灵长类动物细胞，包括人类细胞，可以在足够灵敏的水平上用于基因转移实验，以允许检测单拷贝基因功能。最近，我们也成功地将这种方法用于未分化的胚胎细胞。
We have generalized the protocol of gene transfer, greatly increasing the variety of cells that can be used as recipients of foreign genes. Our approach has been to use a transient assay system that allows rapid screening of expression of foreign DNA. When the initial steps of gene transfer have been optimized with the transient system, these defined conditions are used to yield efficient stable transformation. We have seen that primate cells, including human cells, can be used in gene transfer experiments at levels sensitive enough to allow detection of single copy gene function. Recently we have also used this approach successfully with undifferentiated embryonic cells.