Angiotensin-II receptor subtypes in fetal tissue of the rat: autoradiography, guanine nucleotide sensitivity, and association with phosphoinositide hydrolysis.
Angiotensin-II receptor subtypes in fetal tissue of the rat: autoradiography, guanine nucleotide sensitivity, and association with phosphoinositide hydrolysis.
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大鼠胎儿组织中的血管紧张素-II 受体亚型：放射自显影、鸟嘌呤核苷酸敏感性以及与磷酸肌醇水解的关联。
DOI：
10.1210/endo-129-2-1075
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发表时间：
1991
期刊：
影响因子：
4.8
通讯作者：
J. Saavedra
中科院分区：
文献类型：
--
作者：
K. Tsutsumi;C. Strömberg;M. Viswanathan;J. Saavedra
Quantitative autoradiography revealed large numbers of angiotensin-II (AT) receptors in the 18-day-old rat embryo. The selective AT-1 antagonist DuP 753 readily competed for AT receptors in liver, lung parenchyma, and choroid plexus, and these receptors are classified as AT-1 receptors. The selective AT-2 displacers CGP 42112 A and/or PD 123177 competed with high affinity with AT bound to most receptors located in skeletal muscle, skin, diaphragm, bronchi, and stomach, and these receptors are classified as AT-2 receptors. The amount of AT-2 receptors in fetal tissue was more than 10-fold higher than that of AT-1 receptors. In skeletal muscle and skin, DuP 753 competed with AT in the presence of 10(-7) M CGP 42112 A, indicating the presence of small numbers of AT-1 receptors. In liver and lung parenchyma, binding to AT-1 receptors was sensitive to guanine nucleotides. AT binding to AT-2 receptors in fetal skin and skeletal muscle was insensitive to guanine nucleotides. AT stimulated phosphoinositide hydrolysis in liver (ED50, 64 nM) and in skin and skeletal muscle (ED50, 62 nM); this was inhibited by DuP 753 (liver IC50, 38 nM; skin and skeletal muscle IC50, 26 nM), but not by PD 123177 in concentrations up to the micromolar range. AT-1 receptors are probably coupled to G-proteins, and their stimulation increases phosphoinositide hydrolysis. AT-2 receptors may not be linked to G-proteins, their stimulation is not associated with phosphoinositide hydrolysis, and the nature of their second messenger system(s) is presently unknown.
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DOI：
10.1073/pnas.85.10.3633
发表时间：
1988-05-01
影响因子：
11.1
作者：
EWALD, DA;STERNWEIS, PC;MILLER, RJ
通讯作者：
MILLER, RJ
DOI：
10.1016/0002-9343(88)90201-x
发表时间：
1988
期刊：
The American journal of medicine
影响因子：
--
作者：
Dzau,VJ
通讯作者：
Dzau,VJ
3h-P物质与唾液腺膜结合。
影响因子：
3.6
作者：
Lee,CM;Javitch,JA;Snyder,SH
通讯作者：
Snyder,SH
生长激素释放因子与大鼠垂体受体结合的核苷酸调节。
影响因子：
4.8
作者：
Struthers,RS;Perrin,MH;Vale,W
通讯作者：
Vale,W