Identification of residues in the nucleotide binding site of the epidermal growth factor receptor/kinase.
Identification of residues in the nucleotide binding site of the epidermal growth factor receptor/kinase.
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DOI：
10.1016/s0021-9258(18)89002-0
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发表时间：
1985-05
期刊：
影响因子：
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通讯作者：
M. Russo;T. Lukas;S. Cohen;J. V. Staros
中科院分区：
文献类型：
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作者：
M. Russo;T. Lukas;S. Cohen;J. V. Staros
我们已经从A431膜囊泡中纯化了表皮生长因子受体/激酶，该膜囊泡已经用ATP类似物5 '-对氟磺酰基苯甲酰基[8- 14 C]腺苷进行了亲和标记。将所得纯化的亲和标记的受体/激酶制剂进行还原和羧甲基化，然后进行胰蛋白酶消化。从这个消化，我们已经分离和测序的胰蛋白酶肽含有ATP类似物标记的主要网站。该肽的序列是Ile-Pro-Val-Ala-Ile-X-Glu-Leu，其中X对应于EGF受体/激酶的衍生序列的Lys 721。
We have purified the epidermal growth factor receptor/kinase from A431 membrane vesicles which had been affinity labeled with the ATP analog, 5'-p-fluorosulfonylbenzoyl[8-14C]adenosine. The resulting purified, affinity labeled receptor/kinase preparation has been subjected to reduction and carboxymethylation followed by tryptic digestion. From this digest, we have isolated and sequenced the tryptic peptide containing the major site of labeling by the ATP analog. The sequence of this peptide is Ile-Pro-Val-Ala-Ile-X-Glu-Leu, where X corresponds to Lys 721 of the derived sequence of the EGF receptor/kinase.