Morphological and Functional Evidence for the Contribution of the Pudendal Artery in Aging-Induced Erectile Dysfunction
Morphological and Functional Evidence for the Contribution of the Pudendal Artery in Aging-Induced Erectile Dysfunction
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DOI：
10.1111/j.1743-6109.2010.01920.x
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发表时间：
2010-10-01
影响因子：
3.5
通讯作者：
Adams, Michael A.
中科院分区：
文献类型：
--
作者：
Hannan, Johanna L.;Blaser, Mark C.;Adams, Michael A.
衰老增加了勃起功能障碍（艾德）和心血管疾病的风险。这些条件有相似的病因和共同共存。一个统一的概念是动脉功能不全的作用，这是年龄相关性艾德的发病的主要因素。目的：基于阴部动脉贡献总阴茎血管阻力的70%的新发现，我们的目的是在年轻和老年血压正常的大鼠中，从形态学和功能学上描述该血管的特征。道利大鼠，使用阿扑吗啡生物测定法（80 mg/kg，s.c.）。将麻醉动物灌注固定，切除主动脉、肾动脉和阴部内动脉，包埋、切片、染色，并使用光学显微镜进行形态测定评估。在灌注前切除心脏，分离并称重。收缩和舒张反应乙酰胆碱（ACh）和苯肾上腺素（PE）进行了评估，通过钢丝myograph.Main Outcome Measures.勃起功能，形态学测量，浓度响应曲线ACh和PE.Results.With年龄，有显着下降勃起反应相比，年轻的大鼠（2.8 +/- 0.87 vs. 0.3 +/- 0.58）。阴部动脉的管腔相对较小（303 +/- 13.8 μ m），中层较厚（47 +/- 2.2 μ m）。在老年阴部动脉，管腔直径没有改变，但中层，横截面积和细胞外基质显着增加。相反，老龄大鼠主动脉和肾动脉的管腔直径和壁厚成比例增加。老年阴部动脉中小而圆的平滑肌细胞增加。在功能上，没有差异的收缩反应PE，但是，乙酰胆碱诱导的放松随着年龄的增长而下降。结论：在老年大鼠，勃起功能严重减少时，阴部动脉发生了显着的表型变化。具体而言，随着年龄的增长，该血管出现内皮功能障碍和病理性重塑，其特征为中膜增厚、血管舒张受损和阴茎血流能力显著降低。Hannan JL，Blaser MC，Oldfield L，Pang JJ，亚当斯SM，Pang SC和亚当斯MA。阴部动脉在衰老性勃起功能障碍中作用的形态学和功能学证据。J Sex Med 2010;7：3373-3384.
Introduction.Aging increases the risk of both erectile dysfunction (ED) and cardiovascular disease. These conditions have similar etiologies and commonly coexist. One unifying concept is the role of arterial insufficiency which is a primary factor in the onset of age-related ED.Aim.Based on the novel finding that the pudendal arteries contribute 70% of the total penile vascular resistance, our objective was to morphometrically and functionally characterize this vessel in young and old normotensive rats.Methods.Erectile function was monitored in 15- and 77-week Sprague-Dawley rats using the apomorphine bioassay (80 mg/kg, s.c.). Anesthetized animals were perfusion-fixed, aortic, renal, and internal pudendal arteries were excised, embedded, sectioned, stained, and morphometrically assessed using light microscopy. Hearts were excised, separated, and weighed prior to perfusion. Contractile and relaxation responses to acetylcholine (ACh) and phenylephrine (PE) were assessed by wire myograph.Main Outcome Measures.Erectile function, morphological measurements, concentration response curves to ACh and PE.Results.With age, there were marked decreases in erectile responses compared to younger rats (2.8 +/- 0.87 vs. 0.3 +/- 0.58). The pudendal arteries had a relatively small lumen (303 +/- 13.8 mu m) and a thick medial layer (47 +/- 2.2 mu m). In aged pudendal arteries, the lumen diameter did not change, and yet the medial layer, cross sectional area, and extracellular matrix were markedly increased. In contrast, the lumen diameter and wall thickness of the aorta and renal arteries in aged rats increased proportionally. An increase in small, round, smooth muscle cells was seen in aged pudendal arteries. Functionally, there were no differences in contractile responses to PE; however, ACh-induced relaxation decreased with age.Conclusions.In aged rats, erectile function was severely diminished when pudendal arteries had undergone marked phenotypic changes. Specifically, there was endothelial dysfunction and pathological remodeling of this vessel with age, characterized by medial thickening, impaired vasodilation and significantly reduced capacity for penile blood flow. Hannan JL, Blaser MC, Oldfield L, Pang JJ, Adams SM, Pang SC, and Adams MA. Morphological and functional evidence for the contribution of the pudendal artery in aging-induced erectile dysfunction. J Sex Med 2010;7:3373-3384.