Determination in vivo of newly synthesized gene expression in hamsters during phases of the hibernation cycle
Determination in vivo of newly synthesized gene expression in hamsters during phases of the hibernation cycle
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DOI：
10.2170/jjphysiol.54.295
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Hashimoto, M
中科院分区：
文献类型：
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作者：
Osborne, PG;Gao, B;Hashimoto, M
本研究测定了叙利亚仓鼠（Mesocricetus auratus）在（a）33 h的麻痹（体温56 ℃）;（B）90 min的早期觉醒;（c）90 min的中期觉醒;（d）90 min的冷适应性恒温（CEN）仓鼠在相同的昼夜周期中的皮质、小脑和中脑/脑干以及6个主要器官的总RNA和蛋白质的体内合成。适当的生理参数用于确认在输注和掺入[3 H]尿苷和[C-14]-亮氨酸期间的冬眠周期的阶段。在休眠状态下，RNA的合成是CEN水平的5-25%，这取决于组织。在大脑和心脏的mRNA没有优先合成。蛋白质的合成在低，组织特定的水平在麻木。在早期觉醒过程中，通过非颤抖性产热启动觉醒和前部器官变暖，而没有可测量的RNA或蛋白质合成。组织特异性水平的RNA和蛋白质的合成发生后颤抖产热已被招募，并强烈影响体内的热梯度。在中间唤醒阶段，蛋白质合成在大脑中最活跃，尽管RNA和mRNA的合成量很小。诱导和维持冬眠以及从冬眠中唤醒直到颤抖性产热发作所需的大部分分子加工发生在仓鼠启动冬眠周期之前的恒温期。
This study measured in vivo synthesis of total RNA and protein from cortex, cerebellum and midbrain/brainstem and 6 major organs from Syrian hamsters (Mesocricetus auratus) during (a) 33 h of torpor (body temperature 56 degreesC); (b) 90 min of the early arousal; (c) 90 min of the middle arousal; (d) 90 min in cold adapted cenothermic (CEN) hamsters of the same circannual period. Appropriate physiological parameters were used to confirm the phase of the hibernation cycle during infusion and incorporation of [3 H]uridine and [C-14]-leucine. In torpor, RNA synthesis was 5-25% of CEN levels depending upon tissue. In brain and heart mRNA was not preferentially synthesized. Protein was synthesized at low, tissue specific levels during torpor. Initiation of arousal and the warming of anterior organs via non-shivering thermogenesis during the early arousal occurred without measurable synthesis of RNA or proteins. Tissue specific levels of RNA and protein synthesis occurred later after shivering thermogenesis had been recruited and was strongly influenced by thermal gradients in the body. In the middle arousal phase, protein synthesis is most active in the brain despite modest synthesis of RNA and mRNA. The majority of molecular processing required for the induction and maintenance of torpor and the arousal from torpor up until the onset of shivering thermogenesis occurs during the cenothermic period before the hamster initiates the hibernation cycle.