Association of gut microbial communities with plasma lipopolysaccharide-binding protein (LBP) in premenopausal women
Association of gut microbial communities with plasma lipopolysaccharide-binding protein (LBP) in premenopausal women
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DOI：
10.1038/s41396-018-0064-6
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发表时间：
2018-07-01
期刊：
影响因子：
11
通讯作者：
Hullar, Meredith A. J.
中科院分区：
文献类型：
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作者：
Citronberg, Jessica S.;Curtis, Keith R.;Hullar, Meredith A. J.
肥胖增加癌症风险的机制尚不清楚，但一些证据表明，肠道微生物群落(GMC)可能通过提高全身脂多糖(LPS)水平而导致肥胖者的慢性炎症。我们评估了110名美国绝经前妇女粪便中GMC与血浆内毒素结合蛋白(LBP)和C反应蛋白(CRP)浓度的相关性。饮食使用3天食物记录进行评估，转基因细胞使用16S rRNA基因焦磷酸测序进行评估。OTU的序列相似性为97%。从16S rRNA基因中推算出分类和功能基因，并分别用Shannon指数和MRPP评价α和β多样性。用多元线性回归分析来评估LBP、指示种分析鉴定的特定细菌属和CRP之间的关系。膳食脂肪摄入量，特别是饱和脂肪摄入量和C反应蛋白与LBP增加呈正相关。在使用未加权Unifrac的组之间，GMCβ多样性，而不是阿尔法多样性，在统计上有显著差异。一些分类群，特别是梭状芽胞杆菌类的分类群，在低LBP的女性中更常见，而类杆菌在高LBP的女性中更常见。与革兰氏阴性细胞壁物质合成相关的基因也与LBP和CRP相关。相反，相弧菌与较低浓度的LBP和CRP有关。我们发现不同类型的LBP在微生物组的多样性和组成方面存在明显的差异，并且在可能激活LBP的功能基因上也存在差异。
The mechanisms by which obesity increases cancer risk are unclear, but some lines of evidence suggest that gut microbial communities (GMC) may contribute to chronic inflammation in obese individuals through raised systemic levels of lipopolysaccharides (LPS). We evaluated associations of the GMC in stool with plasma LPS-binding protein (LBP, a measure of LPS) and C-reactive protein (CRP) concentrations in 110 premenopausal women in the United States. Diet was assessed using 3-day food records and GMCs were evaluated using pyrosequencing of the 16S rRNA gene. OTUs were identified at 97% sequence similarity. Taxonomic classification and functional genes were imputed from 16S rRNA genes, and alpha and beta diversity were assessed using the Shannon index and MRPP, respectively. Multivariable linear regression analysis was used to assess the relation between LBP, specific bacterial genera identified with indicator species analysis, and CRP. Dietary fat intake, particularly saturated fat, and CRP were positively associated with increased LBP. GMC beta diversity, but not alpha diversity, was statistically significantly different between groups using unweighted Unifrac. Several taxa, particularly those in the Clostridia class, were more prevalent in women with low LBP, while Bacteroides were more prevalent in those with high LBP. Genes associated with gram-negative cell wall material synthesis were also associated with LBP and CRP. In contrast, Phascolarctobacterium was associated with lower concentrations of LBP and CRP. We found distinct differences between tertiles of LBP regarding the diversity and composition of the microbiome, as well as differences in functional genes that potentially activate LBP.