The rat albumin gene promoter is appropriately regulated in transient but not in stable transfections.
The rat albumin gene promoter is appropriately regulated in transient but not in stable transfections.
复制标题
大鼠白蛋白基因启动子在瞬时转染中受到适当调节，但在稳定转染中不受调节。
DOI：
10.1093/nar/16.24.11573
复制
发表时间：
1988
影响因子：
14.9
通讯作者：
Chasin,LA
中科院分区：
文献类型：
--
作者：
Berland,R;Chasin,LA
The tissue-specific expression of the liver-specific rat albumin gene promoter was examined after transfer to various hepatic and non-hepatic cell lines. A 402 base pair sequence from the albumin gene 5′ flank enabled a fused reporter chloramphenicol acetyltransferase gene to be expressed in rat hepatoma cell lines but not in fibroblast lines or dedifferentiated hepatoma cells. However, when this same construct was analyzed in permanently transfected cell populations, it was expressed equally well in differentiated and dedifferentiated hepatoma cells and in two of three fibroblast lines tested. The inappropriate expression of the albumin promoter was also seen using the HSVtkgene and the E. coligptgene as reporters, and when assayed by colony formation in HAT medium (tkgene) or by S1 protection of transcripts in cotransfected populations (tkandgptgenes). These results show that gene regulatory elements can behave differently in transient vs. stable transfections, and suggest that chromosomal integration can provide long range positive influences on gene expression.
