Callose implication in stomatal opening and closure in the fern Asplenium nidus
Callose implication in stomatal opening and closure in the fern Asplenium nidus
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DOI：
10.1111/j.1469-8137.2010.03206.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Galatis, B.
中科院分区：
文献类型：
--
作者：
Apostolakos, P.;Livanos, P.;Galatis, B.
通过观察愈伤组织在开闭气孔中的沉积模式，以及愈伤组织在气孔运动中的降解和对愈伤组织合成的抑制或诱导作用，研究了愈伤组织在蕨类植物巢状铁角蕨气孔开闭机制中的作用。胼胝质合成被2-脱氧-d-葡萄糖抑制，并被香豆素或氯苄腈诱导。气孔开放和关闭的气孔完全没有胼胝质，而关闭的显示出明显的放射状纤维胼胝质阵列外周壁。后者在胼胝质沉积区域显示局部弯曲，这是开放气孔中不存在的变形。胼胝质降解和胼胝质合成的抑制都降低了气孔在白色光下打开和在黑暗中关闭的能力。在相同条件下，诱导胼胝质合成可显著提高气孔开放度，降低气孔关闭度，说明气孔关闭过程中，外周保卫细胞壁受到强烈的机械应力，胼胝质可能参与气孔运动。
P>The involvement of callose in the mechanism of stomatal pore opening and closing in the fern Asplenium nidus was investigated by examination of the pattern of callose deposition in open and closed stomata, and by examination of the effects of callose degradation and inhibition or induction of callose synthesis in stomatal movement.Callose was identified with aniline blue staining and a callose antibody and degraded via beta-1,3-d-glucanase. Callose synthesis was inhibited with 2-deoxy-d-glucose and induced by coumarin or dichlobenil. Stomatal pore opening and closing were assessed by estimation of the stomatal pore width.The open stomata entirely lacked callose, while the closed ones displayed distinct radial fibrillar callose arrays in the external periclinal walls. The latter displayed local bending at the region of callose deposition, a deformation that was absent in the open stomata. Both callose degradation and inhibition of callose synthesis reduced the stomatal ability to open in white light and close in darkness. By contrast, callose synthesis induction considerably improved stomatal pore opening and reduced stomatal closure in same conditions.The present data revealed that: during stomatal closure the external periclinal guard cell walls experience a strong mechanical stress, probably triggering callose synthesis; and that callose participates in stomatal movement.