Short-chain fatty acids, acetate and propionate, directly upregulate osteoblastic differentiation
Short-chain fatty acids, acetate and propionate, directly upregulate osteoblastic differentiation
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短链脂肪酸，乙酸盐和丙酸盐，直接上调成骨细胞分化
DOI：
10.1080/09637486.2022.2078285
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发表时间：
2022-05-26
影响因子：
3.9
通讯作者：
Tousen, Yuko
中科院分区：
文献类型：
--
作者：
Kondo, Takashi;Chiba, Tsuyoshi;Tousen, Yuko
短链脂肪酸，包括乙酸盐、丙酸盐和丁酸盐，是大肠微生物群产生的膳食纤维的代谢产物，已被认为有助于影响骨稳态。然而，尚不清楚它们是否用于成骨细胞并直接影响骨形成。我们使用 MC3T3-E1 细胞研究了短链脂肪酸是否被成骨细胞吸收并影响早期成骨细胞分化。乙酸盐和丙酸盐上调碱性磷酸酶活性（这是一种成骨细胞分化标志物），并且在治疗 9 天后，乙酸盐上调碱性磷酸酶 mRNA 表达，而丁酸盐在 MC3T3-E1 细胞中则不会。丁酸盐比乙酸盐和丙酸盐吸收更快、程度更大。这些结果表明，短链脂肪酸被用于成骨细胞，特别是乙酸盐和丙酸盐直接上调原代成骨细胞的分化。因此，乙酸盐和丙酸盐可能有助于维持骨转换的正平衡。
Short-chain fatty acids, including acetate, propionate, and butyrate are metabolites of dietary fibre produced by microbiota in the large intestine, have been proposed to contribute to effects on bone homeostasis. However, it is unclear whether they are used in osteoblasts and directly affect bone formation. We investigated whether short-chain fatty acids are absorbed in osteoblast cells and influence early osteoblastic differentiation using MC3T3-E1 cells. Acetate and propionate upregulated alkaline phosphatase activity, which is an osteoblast differentiation marker, and acetate upregulated alkaline phosphatase mRNA expression after treatment for 9 days, whereas butyrate did not in MC3T3-E1 cells. Butyrate was absorbed more rapidly and to a greater extent than acetate and propionate. These results indicate that short-chain fatty acids were used in osteoblastic cells, and particularly acetate and propionate directly upregulated differentiation in primary osteoblasts. Therefore, acetate and propionate might be useful for maintaining a positive balance of bone turnover.