Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell
Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell
复制标题
DOI：
10.1046/j.1365-2958.2001.02691.x
复制
发表时间：
2001-12-01
影响因子：
3.6
通讯作者：
Nanninga, N
中科院分区：
文献类型：
--
作者：
den Blaauwen, T;Lindqvist, A;Nanninga, N
针对MukB的荧光多克隆抗体已被用于研究其在大肠杆菌中的定位。在野生型细胞中，MukB蛋白表现为有限数量的椭圆形形状，包围着类核。MukB在没有DNA复制的情况下仍然与类核结合。分散的MukB信号的重心最初定位在中细胞附近，但在DNA复制终止及其随后的重新启动之前移动到大约四分之一的位置。由于MukB已被报道在体外结合FtsZ和其真核同源微管蛋白，细胞用MukB和FtsZ特异性荧光团共标记。在细胞周期的任何时间都没有观察到MukB与聚合的FtsZ（FtsZ环）的共定位。MukB在防止过早FtsZ聚合和DNA折叠，可能有助于DNA分离的可能作用进行了讨论。
Fluorescent polyclonal antibodies specific for MukB have been used to study its localization in Escherichia coli. In wild-type cells, the MukB protein appeared as a limited number of oblong shapes embracing the nucleoid. MukB remained associated with the nucleoid in the absence of DNA replication. The centre of gravity of the dispersed MukB signal initially localized near mid-cell, but moved to approximately quarter positions well before the termination of DNA replication and its subsequent reinitiation. Because MukB had been reported to bind to FtsZ and to its eukaryotic homologue tubulin in vitro, cells were co-labelled with MukB- and FtsZ-specific fluorophores. No co-localization of MukB with polymerized FtsZ (the FtsZ ring) was observed at any time during the cell cycle. A possible role for MukB in preventing premature FtsZ polymerization and in DNA folding that might assist DNA segregation is discussed.