Combination of label-free quantitative proteomics and transcriptomics reveals intraspecific venom variation between the two strains of Tetrastichus brontispae, a parasitoid of two invasive beetles.
Combination of label-free quantitative proteomics and transcriptomics reveals intraspecific venom variation between the two strains of Tetrastichus brontispae, a parasitoid of two invasive beetles.
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DOI：
10.1016/j.jprot.2018.08.003
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发表时间：
2019-02
影响因子：
3.3
通讯作者：
B. Tang;E. Meng;Huajian Zhang;Xiao-Mei Zhang;Sassan Asgari;Yaping Lin;Yun-Ying Lin;Z. Peng
中科院分区：
文献类型：
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作者：
B. Tang;E. Meng;Huajian Zhang;Xiao-Mei Zhang;Sassan Asgari;Yaping Lin;Yun-Ying Lin;Z. Peng
毒液器是寄生蜂的一个保守的器官，显示出与生活方式多样化相关的适应性。本研究结合转录组学和无标记定量蛋白质组学技术，对椰心叶甲啮小蜂Tetrastichus brontispae（Eulophidae）的毒液器官组分进行了研究，并对两个近缘品系的毒液器官蛋白质组进行了比较。brontispae-acridonta nitriana（Tb-On）和T.椰心叶甲-椰心叶甲（Brontispa longissima，Tb-B1）。TB-B1靶向B. longissimapupa作为其习惯性宿主。然而，Tb-On是Tb-Bl的实验衍生物，其已经暴露于O。nipaepupa连续40余代。结果表明，在中华绒螯蟹中鉴定出约1505种毒液蛋白。椰心叶甲提取物含有新的毒液蛋白，如4-香豆酸-CoA连接酶4。毒液蛋白质组的比较分析表明，毒液的组成存在显着的定量和定性差异，两种菌株之间，虽然最丰富的毒液蛋白质之间共享。差异产生的蛋白质主要富集在脂肪酸的生物合成和黑色素包裹反应。这些富集的蛋白质中有六种在Tb-On中呈现出增加的水平，并且该结果通过平行反应监测（PRM）分析进行了验证。总的来说，我们的数据表明，毒液成分可以迅速进化，并对宿主选择做出反应。
The venom apparatus is a conserved organ in parasitoids that shows adaptations correlated with life-style diversification. Combining transcriptomics and label-free quantitative proteomics, here we explored the venom apparatus components of the endoparasitoidTetrastichus brontispae(Eulophidae), and provide a comparison of the venom apparatus proteomes between its two closely related strains,T. brontispae-Octodonta nipae(Tb-On) andT. brontispae-Brontispa longissima(Tb-Bl). Tb-Bl targets theB. longissimapupa as its habitual host. However, Tb-On is an experimental derivative of Tb-Bl, which has been exposed to theO. nipaepupa as host consecutively for over 40 generation. Results showed that approximately 1505 venom proteins were identified in theT. brontispaevenom apparatus. The extracts contained novel venom proteins, such as 4-coumarate-CoA ligase 4. A comparative venom proteome analysis revealed that significant quantitative and qualitative differences in venom composition exist between the two strains; although the most abundant venom proteins were shared between them. The differentially produced proteins were mainly enriched in fatty acid biosynthesis and melanotic encapsulation response. Six of these enriched proteins presented increased levels in Tb-On, and this result was validated by parallel reaction monitoring (PRM) analysis. Overall, our data reveal that venom composition can evolve quickly and respond to host selection.