CHANGES IN GLUTATHIONE IN INTACT ERYTHROCYTES DURING INCUBATION WITH PENICILLAMINE AS DETECTED BY H-1 SPIN-ECHO NMR-SPECTROSCOPY
CHANGES IN GLUTATHIONE IN INTACT ERYTHROCYTES DURING INCUBATION WITH PENICILLAMINE AS DETECTED BY H-1 SPIN-ECHO NMR-SPECTROSCOPY
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DOI：
10.1016/0167-4889(86)90067-4
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发表时间：
1986-08-29
期刊：
影响因子：
--
通讯作者：
STURROCK, RD
中科院分区：
文献类型：
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作者：
MCKAY, CNN;BROWN, DH;STURROCK, RD
用~1H自旋回波核磁共振波谱研究完整红细胞谷胱甘肽状态的变化。类风湿关节炎患者红细胞在2H2O生理盐水中悬浮液中谷胱甘肽的浓度与正常健康志愿者的红细胞悬浮液中的谷胱甘肽浓度有显著差异。观察到分离和悬浮的方法会影响红细胞中的乳酸代谢。红细胞与治疗剂D-青霉胺溶液在2H2O生理盐水中孵育产生谷胱甘肽共振的变化，这表明二谷硫酮浓度增加。来自青霉胺甲基的信号以相称的速度减少。将正常细胞与与正常志愿者血型相同的类风湿性关节炎患者的血浆孵育显示，接受青霉胺治疗的患者血浆中谷胱甘肽信号的下降幅度比服用非类固醇消炎药的患者大得多。
1H spin-echo NMR spectroscopy was used to study changes in glutathione status in intact erythrocytes. The concentration of glutathione in suspensions of erythrocytes in 2H2O saline is significantly different in cells from patients with rheumatoid arthritis than in controls from normal healthy volunteers. It is observed that the methods of separation and suspension affect lactate metabolism in red cells. Incubation of erythrocytes with solutions of the therapeutic agent D-penicillamine in 2H2O saline produced a change in glutathione resonances which is indicative of an increase in diglutathione concentration. Signals from the methyl groups of penicillamine decreased at a commensurate rate. Incubation of normal cells with plasma from patients with rheumatoid arthritis who had the same blood group as the normal volunteer indicated a much larger fall in glutathione signal with plasma from a patient treated with penicillamine than from a patient on non-steroidal anti-inflammatories.