Clinical Impact of the Immune Microenvironment in Spinal Chordoma: Immunoscore as an Independent Favorable Prognostic Factor
Clinical Impact of the Immune Microenvironment in Spinal Chordoma: Immunoscore as an Independent Favorable Prognostic Factor
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DOI：
10.1093/neuros/nyy274
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发表时间：
2019-06-01
期刊：
影响因子：
4.8
通讯作者：
She, Xiao-Ling
中科院分区：
文献类型：
--
作者：
Zou, Ming-Xiang;Lv, Guo-Hua;She, Xiao-Ling
目的研究免疫细胞在脊索瘤中的原位表达特征，并探讨其与脊索瘤患者临床病理资料及预后的关系。方法采用免疫组织化学方法检测54例脊索瘤标本中CD3、CD4、CD8、CD20、Foxp3、PD-1、PD-L1的表达。肿瘤内(TI)区的PD-1(+)TIL密度、CD3(+)、CD8(+)TIL密度与肿瘤侵犯周围肌肉呈正相关，而PD-L1(+)TIL与肿瘤病理分级、分期呈负相关。肿瘤组织中PD-1(+)TIL、PD-L1(+)TIL、CD4(+)TIL和CD3(+)TIL的密度与局部无复发生存期和总生存期(OS)显著相关。而Foxp3(+)TIL(P=.024)和CD8(+)TIL在TI(P
BACKGROUND Currently, clinical implications of immune system cells in chordoma remain to be elucidated.OBJECTIVE To characterize in situ immune cell infiltrates, the Immunoscore, and investigate their correlation with clinicopathologic data of spinal chordoma patients and outcome.METHODS Tumor-infiltrating lymphocytes (TILs) subtypes were assessed in 54 tumor specimens using immunohistochemistry for CD3, CD4, CD8, CD20, Foxp3, PD-1, and PD-L1.RESULTS Overall, immune cell infiltrates were present in all samples and there was low or moderate correlation among several TILs subsets. PD-1(+) TILs density, CD3(+), and CD8(+) TILs densities in the tumor interior (TI) subarea were associated with surrounding muscle invasion by tumor, whereas PD-L1(+) TILs showed inverse association with tumor pathological grade and stage. The density of PD-1(+) TILs, PD-L1(+) TILs, CD4(+) TILs, and CD3(+) TILs both in the TI and combined tumor regions (TI and invasion margin) were significantly associated with local recurrence-free survival and overall survival (OS). However, Foxp3(+) TILs (P=.024) and CD8(+) TILs evaluated in the TI (P