Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection
Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection
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DOI：
10.1117/1.2437143
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发表时间：
2007-01-01
影响因子：
3.5
通讯作者：
Zappe, Hans
中科院分区：
文献类型：
--
作者：
Glasenapp, Carsten;Moench, Wolfgang;Zappe, Hans
描述了一种高度并行的微阵列扫描仪，用于功能基因组研究，使用动态可重构全息激发和高光谱荧光检测。利用一对空间光调制器和一种新型成像系统，将来自两个激光源（405 nm和532 nm）的光在生物芯片上分成任意数量的聚焦点。并行光学扫描仪包括一个高光谱检测单元和一个高灵敏度CCD相机，可以同时检测和分析所有照射点的发射荧光光谱（从430到800 nm）。通过使用xy扫描仪，可以逐行读出整个阵列上所有点的光谱。(c) 2007年光学仪器工程师学会。
A highly parallel microarray scanner for functional genomic research using a dynamically reconfigurable holographic excitation and hyperspectral fluorescence detection is described. The light from two laser sources (405 and 532 nm) is split into an arbitrary number of focused spots on the biochip using a pair of spatial light modulators and a novel imaging system. The parallel optical scanner includes a hyperspectral detection unit with a high-sensitivity CCD camera to detect and analyze the emitted fluorescence spectra (from 430 to 800 nm) of all illuminated spots simultaneously. By using an xy scanner, the spectra of all spots on an entire array can be read out line by line. (c) 2007 Society of Photo-Optical Instrumentation Engineers.